Abstract |
Two endogenous antioxidants that are speculated to be defense substances against preeclampsia, 2-hydroxyestradiol (2-OH-E2) and its 17-sulfate, 2-hydroxyestradiol 17-sulfate (2-OH-E2-17-S), were administered to rats to compare their inhibitory effects on hepatic microsomal lipid peroxidation, and the lipid peroxides were determined in NADPH- and ascorbic acid-dependent systems. The two catechols showed a strong inhibitory effect on lipid peroxidation in both systems, and the effect was dose dependent. However, a large difference was observed in their inhibition patterns. After administration of 2-OH-E2, the effect appeared immediately and decreased gradually with time. In contrast, the effect of 2-OH-E2-17-S appeared some time after administration and persisted for a longer time. Both catechols also showed a striking difference in their dynamics. After administration, 2-OH-E2 was detected in the blood together with its metabolites, 2-methoxyestradiol and 2-methoxyestrone, and they disappeared immediately. In contrast, 2-OH-E2-17-S was present in the blood for a longer time together with its O-methylated product, 2-methoxyestradiol 17-sulfate, but disappeared from liver microsomes within 2 h after administration. The results imply no occurrence of a direct inhibition effect of 2-OH-E2-17-S.
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Authors | Kaori Takanashi, Yasunori Osanai, Takahiro Kyo, Itsuo Yoshizawa |
Journal | Lipids
(Lipids)
Vol. 38
Issue 8
Pg. 847-54
(Aug 2003)
ISSN: 0024-4201 [Print] United States |
PMID | 14577664
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Estrogens
- Estradiol
- 2-hydroxyestradiol 17-sulfate
- 2-hydroxyestradiol
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Topics |
- Animals
- Dose-Response Relationship, Drug
- Estradiol
(analogs & derivatives, blood, pharmacology)
- Estrogens
(blood, pharmacology)
- Lipid Peroxidation
(drug effects)
- Liver
(drug effects)
- Male
- Microsomes, Liver
(drug effects, metabolism)
- Models, Molecular
- Molecular Structure
- Rats
- Rats, Wistar
- Time Factors
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