Effects of thalidomide on parameters involved in angiogenesis: an in vitro study.

Abstract

In an attempt to elucidate the mechanism(s) of action of thalidomide, a reportedly antiangiogenic molecule recently tested in the treatment of relapsing malignant gliomas, we performed an in vitro study on the following parameters: (a) effect of thalidomide on proliferation of endothelial cells; (b) effect of thalidomide on expression of alpha(v)beta3 integrin on the surface of endothelial cells; (c) effect of thalidomide on the release by endothelial cells of MMP-2, IL-8 and TNF-alpha. The results show that thalidomide inhibits endotelial cell proliferation induced by bFGF and VEGF, more so if the cells are grown on vitronectin; moreover, treatment with thalidomide reduces the release of MMP-2 and IL-8 by endothelial cells, suggesting a further pathway for the antiangiogenic activity of drug. On the other hand, thalidomide does not modify expression of alpha(v)beta3 on endothelial cells.

• Authors: M Gelati, E Corsini, S Frigerio, B Pollo, G Broggi, D Croci, A Silvani, A Boiardi, A Salmaggi
• Journal: Journal of neuro-oncology (J Neurooncol) Vol. 64 Issue 3 Pg. 193-201 (Sep 2003) ISSN: 0167-594X [Print] United States
• PMID: 14558594 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inhibitors
• Endothelial Growth Factors
• Integrin alphaVbeta3
• Intercellular Signaling Peptides and Proteins
• Interleukin-8
• Lymphokines
• Receptors, Cell Surface
• Tumor Necrosis Factor-alpha
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Vitronectin
• Fibroblast Growth Factor 2
• Thalidomide
• Matrix Metalloproteinase 2

Topics

• Angiogenesis Inhibitors (pharmacology)
• Cell Division (drug effects)
• Cells, Cultured
• Endothelial Growth Factors (physiology)
• Endothelium, Vascular (cytology, drug effects, growth & development, metabolism)
• Fibroblast Growth Factor 2 (physiology)
• Humans
• Integrin alphaVbeta3 (metabolism)
• Intercellular Signaling Peptides and Proteins (physiology)
• Interleukin-8 (metabolism)
• Lymphokines (physiology)
• Matrix Metalloproteinase 2 (metabolism)
• Neovascularization, Pathologic (prevention & control)
• Neovascularization, Physiologic (drug effects)
• Receptors, Cell Surface (metabolism)
• Thalidomide (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• Umbilical Veins (cytology)
• Vascular Endothelial Growth Factor A
• Vascular Endothelial Growth Factors
• Vitronectin (physiology)