Abstract | OBJECTIVE: To explore the relation between H. pylori infection and the development of gastric mucosa lesions, and to evaluate the effect of H. pylori infection on cell proliferation, p53 mutation, and MG-7 antigen expression in patients with gastric precancerous lesions. METHODS: One hundred and nine gastric biopsy specimens were divided into five groups of different gastric mucosa lesions according to pathologic findings and the results of mucosa histochemical staining: type I intestinal metaplasia (IM), type IIIM, type III IM, dysplasia (Dys) and gastric cancer (GC). H. pylori and its cagA status were assessed by microdissection/PCR method. Expression of P53 protein and MG-7 antigen were examined by immunohistochemical staining. AgNOR staining was performed for each specimen. RESULTS: (1) Expression of P53 increased in more severe gastric mucosa lesion groups. The P53 expression rate in GC group was significantly higher than other groups (P < 0.05). There was no difference in expression of P53 between groups of different H. pylori status as while as different cagA status. (2) The expression of MG-7 in GC group was significantly higher than that in other groups (P < 0.05). There were no difference of expression of MG-7 between subgroups of different H. pylori infection status or cagA status in all groups (P > 0.05). (3) The counts of argyrophil protein of the nucleolar organizer regions ( AgNORs) were significantly increased in more severe gastric mucosa lesion groups (P < 0.05), and were significantly higher in H. pylori positive and cagA positive specimens than in other specimens (P < 0.05). CONCLUSIONS:
Infection with H. pylori, particularly cagA-positive strains, is associated with the development of more severe gastric mucosa lesions; it seems to have effects on cell proliferation in patients with gastric precancerous lesions. Expression of P53 and MG-7 are earlier events in gastric cancer carcinogenesis.
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Authors | Gui-bin Yang, Fu-lian Hu, You-yong Lü |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 83
Issue 15
Pg. 1331-5
(Aug 10 2003)
ISSN: 0376-2491 [Print] China |
PMID | 12930689
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adult
- Aged
- Antigens, Neoplasm
(analysis)
- Female
- Gastric Mucosa
(pathology)
- Genes, p53
- Helicobacter Infections
(complications)
- Helicobacter pylori
- Humans
- Male
- Middle Aged
- Mutation
- Nucleolus Organizer Region
(ultrastructure)
- Stomach Neoplasms
(etiology, genetics, immunology)
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