Abstract | OBJECTIVE: To determine the effect of NOS2 gene deletion on articular cartilage degradation in autoantibody mediated arthritis (AMA). METHODS: RESULTS: Both NOS2-/- and WT mice with AMA developed clinically apparent arthritis. In WT mice, the arthritis progressed rapidly and reached the peak score 11.4 +/- 2.9 on Day 12, whereas the arthritis in NOS2-/- mice was milder and the peak score was 7.7 +/- 2.8 on Day 13 (p < 0.05). The serum nitrite/ nitrate levels, histological grades of articular cartilage degradation, and numbers of apoptotic chondrocytes and nitrotyrosine positive chondrocytes were significantly lower in NOS2-/- mice with AMA than in WT mice with AMA. Conversely, significant differences were not observed in MMP-3 or -9 expression in chondrocytes, or in serum IL-1 beta levels between these 2 groups of mice. CONCLUSION: NOS2 gene deletion did not affect the inflammatory responses, but reduced the cartilage degradation.
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Authors | Hisayoshi Kato, Keiichiro Nishida, Aki Yoshida, Itsuro Takada, Cherie McCown, Masatsugu Matsuo, Takuro Murakami, Hajime Inoue |
Journal | The Journal of rheumatology
(J Rheumatol)
Vol. 30
Issue 2
Pg. 247-55
(Feb 2003)
ISSN: 0315-162X [Print] Canada |
PMID | 12563676
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Autoantibodies
- Collagen Type II
- Interleukin-1
- Lipopolysaccharides
- Nitrates
- Nitrites
- 3-nitrotyrosine
- Tyrosine
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type II
- Nos2 protein, mouse
- Matrix Metalloproteinase 3
- Matrix Metalloproteinase 9
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Topics |
- Animals
- Antibodies, Monoclonal
(pharmacology)
- Arthritis, Experimental
(immunology, pathology, physiopathology)
- Autoantibodies
(blood)
- Cartilage, Articular
(immunology, pathology)
- Chondrocytes
(enzymology, pathology)
- Collagen Type II
(immunology)
- Disease Models, Animal
- Female
- In Situ Nick-End Labeling
- Interleukin-1
(blood)
- Lipopolysaccharides
(pharmacology)
- Matrix Metalloproteinase 3
(metabolism)
- Matrix Metalloproteinase 9
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Mutant Strains
- Nitrates
(blood)
- Nitric Oxide Synthase
(genetics)
- Nitric Oxide Synthase Type II
- Nitrites
(blood)
- Tyrosine
(analogs & derivatives, metabolism)
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