Levels of mature cross-links and advanced glycation end product cross-links in human vitreous.

Abstract

PURPOSE:

To determine the levels of pyridinoline and deoxypyridinoline, two mature enzymatic cross-links, and pentosidine, an advanced glycation end product (AGE) cross-link, in the human vitreous, and to investigate the correlations among the cross-links and the effects of aging and diabetes mellitus (DM) on the levels of cross-links.

METHODS:

Forty-five vitreous samples were collected from 32 patients (32 eyes) undergoing vitrectomy for diabetic retinopathy (DM group) and from 13 patients (13 eyes) (control group) who were age- and sex-matched patients with idiopathic macular hole or epiretinal membrane with no systemic conditions. The levels of the cross-links were determined using high-performance liquid chromatography after acid hydrolysis and pretreatment with SP-Sephadex.

RESULTS:

The levels of pentosidine, pyridinoline, and deoxypyridinoline were 27.3 +/- 23.1 (mean +/- SD) pmol/mL (detectable in 45 of 45 specimens), 79.0 +/- 40.2 ng/mL (43 of 45 specimens), and 54.0 +/- 9.5 (32 of 45 specimens) ng/mL, respectively. When the vitreous samples from the DM and the control groups were compared, a significant difference (P <.05) was found in the pentosidine level but not in the levels of pyridinoline or deoxypyridinoline. No significant correlations were found between age and the cross-links. Significant correlations (P <.01) were found among the cross-links.

CONCLUSIONS:

The results indicate that mature cross-link substances exist in the human vitreous. The results also suggest that glycation may occur in the vitreous after mature cross-links form and result in the formation of AGE cross-links. In human vitreous from patients with DM, increased levels of AGE cross-links may stabilize the formation of mature cross-links, but they did not increase the mature cross-links.

• Authors: Yukihiro Matsumoto, Masaaki Takahashi, Makoto Chikuda, Kiyomi Arai
• Journal: Japanese journal of ophthalmology (Jpn J Ophthalmol) 2002 Sep-Oct Vol. 46 Issue 5 Pg. 510-7 ISSN: 0021-5155 [Print] Japan
• PMID: 12457909 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Glycation End Products, Advanced
• pyridinoline
• deoxypyridinoline
• Arginine
• pentosidine
• Lysine

Topics

• Adult
• Aged
• Aging (metabolism)
• Amino Acids (metabolism)
• Arginine (analogs & derivatives, metabolism)
• Diabetic Retinopathy (metabolism)
• Glycation End Products, Advanced (metabolism)
• Humans
• Lysine (analogs & derivatives, metabolism)
• Middle Aged
• Reference Values
• Vitreous Body (metabolism)