Type-1 transforming growth factor-beta differentially modulates tumoricidal activity of murine peritoneal macrophages against metastatic variants of the B16 murine melanoma.

Abstract

Transforming growth factor-beta 1 (TGF-beta 1) renders mouse peritoneal macrophages tumoricidal against metastatic variants of the B16 mouse melanoma in vitro. Both direct cytotoxicity and indirect cytotoxicity were observed. A subthreshold concentration (10 U/ml) of recombinant murine interferon-gamma (rMuIFN-gamma) enhanced the direct tumoricidal activity of TGF-beta 1-activated macrophages from 29% to 88% but did not change their indirect tumoricidal profile. Data obtained from macrophages preincubated with either TGF-beta 1 or rMuIFN-gamma showed that TGF-b1 can initiate tumoricidal activity better than rMuIFN-gamma. These effects were plasma-membrane mediated because targeting macrophages with liposomal TGF-beta 1 was ineffective. The order of tumoricidal susceptibility of the B16 melanoma lines to activated macrophages was B16F1 > B16F10 > B16BL6, in inverse order of metastatic potential.

• Authors: Dominic Fan, Amy Liaw, Yvonne M Denkins, James H Collins, Melissa Van Arsdall, Jolie Lien Chang, Subhas Chakrabarty, Dieuthu Nguyen, Ewa Kruzel, Isaiah J Fidler
• Journal: Journal of experimental therapeutics & oncology (J Exp Ther Oncol) 2002 Sep-Oct Vol. 2 Issue 5 Pg. 286-97 ISSN: 1359-4117 [Print] United States
• PMID: 12416032 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Liposomes
• Tgfb1 protein, mouse
• Transforming Growth Factor beta
• Transforming Growth Factor beta1

Topics

• Animals
• Cytotoxicity, Immunologic (drug effects)
• Liposomes
• Macrophages, Peritoneal (immunology)
• Melanoma, Experimental (immunology, therapy)
• Mice
• Mice, Inbred C57BL
• Transforming Growth Factor beta (administration & dosage, pharmacology)
• Transforming Growth Factor beta1
• Tumor Cells, Cultured