Abstract |
In the present paper, we investigated whether the phenotype, the antigen-presenting capacity, and the migration of antigen-presenting cells (APCs), are affected by the aging process. APCs were obtained incubating peritoneal monocyte-macrophage cells with granulocyte-macrophage colony-stimulating factor ( GM-CSF) (immature APCs) or GM-CSF and IFNgamma (mature APCs). Phenotypically, after 8 days incubation, APCs cultures were composed of CD11c and Mac-3 cells, with a similar representation, both in young and old mice. The absolute number and the expression of MHC I and II, CD80, and CD86 both on immature and mature APCs were not significantly different in young and old mice. APCs from old mice induced similar lymphocyte proliferative responses but lower lymphocyte cytotoxicity and a reduced number of CD8(+) T cells producing IFNgamma in comparison with APCs from young animals. Lymphocyte responses were antigen-specific, since TS/A pulsed APCs induced lymphocyte cytotoxicity against TS/A but not against syngeneic TUBO tumor cells. The low expression of the mRNA for the migratory CCR7 chemokine receptor present in immature APCs from old mice was greatly increased in mature APCs up to the levels found in APCs from young animals. The in vivo migration of APCs was higher in old than in young mice. These results demonstrate that some alterations in APCs function are present in aging, suggesting that an increased migratory capacity of old APCs may be required to balance their reduced antigen presentation to cytotoxic lymphocytes.
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Authors | Alessia Donnini, Katy Argentati, Romina Mancini, Arianna Smorlesi, Beatrice Bartozzi, Giovanni Bernardini, Mauro Provinciali |
Journal | Experimental gerontology
(Exp Gerontol)
2002 Aug-Sep
Vol. 37
Issue 8-9
Pg. 1097-112
ISSN: 0531-5565 [Print] England |
PMID | 12213560
(Publication Type: Journal Article)
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Chemical References |
- Ccr7 protein, mouse
- Receptors, CCR7
- Receptors, Chemokine
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Topics |
- Aging
(immunology)
- Animals
- Antigen-Presenting Cells
(physiology)
- Cell Movement
- Cytotoxicity, Immunologic
- Immunophenotyping
- Lymphocyte Activation
- Male
- Mice
- Mice, Inbred BALB C
- Phagocytosis
- Receptors, CCR7
- Receptors, Chemokine
(genetics)
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