HEMA bound to self-protein promotes auto-antibody production in mice.

Abstract	While several studies report that acrylic monomers contained in dental materials may cause hypersensitivity reactions, little is known of the associated immune response. Here we address the potential of 2-hydroxyethyl-methacrylate (HEMA) to bind to endogenous protein and elicit auto-antibody production in vivo. Albumin was incubated with HEMA at various times and pH. Following confirmation of the conjugation by inhibition of trinitrophenyl (TNP) binding, female Balb/c mice received HEMA conjugated to mouse serum albumin (MSA) in Freund's incomplete adjuvant or saline subcutaneously. ELISA was used to determine the serum antibody responses to native and modified MSA. IL-2 production in spleen cell cultures stimulated with HEMA-conjugated MSA was measured. HEMA reacted with serum albumin at physiological conditions. HEMA-conjugated MSA induced IL-2 secretion and production of IgG antibodies to native MSA. The results suggest that modification of an endogenous protein like serum albumin with HEMA may defeat the control of immune responses to this self-protein.
Authors	E Sandberg, G Bergenholtz, C Eklund, U I Dahlgren
Journal	Journal of dental research (J Dent Res) Vol. 81 Issue 9 Pg. 633-6 (Sep 2002) ISSN: 0022-0345 [Print] United States
PMID	12202646 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Autoantibodies Biocompatible Materials Immunoglobulin G Interleukin-2 Methacrylates Serum Albumin Sodium Chloride hydroxyethyl methacrylate Freund's Adjuvant
Topics	Animals Autoantibodies (biosynthesis, blood) Biocompatible Materials (chemistry, pharmacology) Cell Culture Techniques Enzyme-Linked Immunosorbent Assay Female Freund's Adjuvant Hydrogen-Ion Concentration Immunization Immunoglobulin G (biosynthesis) Interleukin-2 (biosynthesis) Lymphocyte Activation (immunology) Methacrylates (chemistry, pharmacology) Mice Mice, Inbred BALB C Protein Binding Serum Albumin (chemistry, immunology) Sodium Chloride Spleen (cytology, immunology) Statistics, Nonparametric T-Lymphocytes (immunology) Time Factors

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