Abstract |
We have studied the inhibitory effect of aloe emodin on hepatic stellate cells activation and proliferation, as these cells play a key role in the pathogenesis of hepatic fibrosis. Rat hepatic stellate cells were activated by contact with plastic dishes, resulting in their transformation into myofibroblast-like cells. Primary hepatic stellate cells were exposed to aloe emodin (1-10 microg/ml). Possible cytotoxic effects were measured on stellate cells and hepatocytes using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of aloe emodin on production of type I collagen and smooth muscle cell alpha-actin were examined at the same concentration, by quantitative immunoprecipitation. Antiproliferative effects were examined by bromodeoxyuridine incorporation. Aloe emodin at 10 microg/ml restored the morphological changes characteristic of activated primary stellate cells, reduced DNA synthesis to 95% of control hepatic stellate cells at 10 microg/ml without affecting cell viability, and inhibited type I collagen production and smooth muscle alpha-actin expression by 86.77% and 99%, respectively, which suggest that aloe emodin is a potent inhibitor of stellate cell transformation.
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Authors | Sun Wook Woo, Ji-Xing Nan, Sung Hee Lee, Eun-Jeon Park, Yu Zhe Zhao, Dong Hwan Sohn |
Journal | Pharmacology & toxicology
(Pharmacol Toxicol)
Vol. 90
Issue 4
Pg. 193-8
(Apr 2002)
ISSN: 0901-9928 [Print] Denmark |
PMID | 12076313
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Anthraquinones
- Collagen Type I
- Enzyme Inhibitors
- Formazans
- Tetrazolium Salts
- MTT formazan
- DNA
- aloe emodin
- Bromodeoxyuridine
- Emodin
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Topics |
- Actins
(metabolism)
- Animals
- Anthraquinones
- Bromodeoxyuridine
(metabolism)
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Cell Survival
(drug effects)
- Collagen Type I
(metabolism)
- DNA
(biosynthesis)
- Dose-Response Relationship, Drug
- Emodin
(pharmacology)
- Enzyme Inhibitors
(pharmacology)
- Fibroblasts
(cytology, drug effects, metabolism)
- Formazans
(metabolism)
- Kupffer Cells
(cytology, drug effects, metabolism)
- Male
- Muscle, Smooth
(cytology, drug effects, metabolism)
- Rats
- Rats, Sprague-Dawley
- Tetrazolium Salts
(metabolism)
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