Abstract | PURPOSE: This study investigated the roles of moderate hypothermia and extraluminal oxygenated perfluorcarbon (PFC) on intestinal metabolism after ischemia-reperfusion. METHODS: A model of 30-minute intestinal ischemia followed by 60 minutes of reperfusion was used. The animals were maintained at either normothermia (36.5 to 37.5 degrees C) or moderate hypothermia (31 to 32 degrees C). Four groups of adult rats were studied (n = 8 per group): (A) sham at normothermia, (B) ischemia-reperfusion at normothermia, (C) ischemia-reperfusion at hypothermia and, (D) ischemia-reperfusion with extraluminal oxygenated PFC perfusion during ischemia at normothermia. Intestinal phosphocreatine, ATP and lactate levels were measured. Histologic changes in the intestine were evaluated. RESULTS: Intestinal ischemia-reperfusion at normothermia caused a marked reduction in phosphocreatine and ATP with an increase in lactate. Moderate hypothermia exerted beneficial effects by attenuating the depletion of high-energy phosphates and the elevation of lactate. Extraluminal PFC perfusion during ischemia failed to produce a protective effect on high-energy phosphates, although it reduced lactate accumulation. Moderate hypothermia significantly decreased the degree of mucosal damage. CONCLUSIONS:
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Authors | Paisarn Vejchapipat, Edward Proctor, Alan Ramsay, Andy Petros, David G Gadian, Lewis Spitz, Agostino Pierro |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 37
Issue 5
Pg. 786-90
(May 2002)
ISSN: 1531-5037 [Electronic] United States |
PMID | 11987102
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2002, Elsevier Science (USA). All rights reserved. |
Chemical References |
- Fluorocarbons
- Phosphocreatine
- Lactic Acid
- Adenosine Triphosphate
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Topics |
- Adenosine Triphosphate
(metabolism)
- Animals
- Energy Metabolism
- Fluorocarbons
(administration & dosage)
- Hypothermia, Induced
- Ileum
(pathology)
- Intestinal Mucosa
(metabolism)
- Lactic Acid
(metabolism)
- Male
- Phosphocreatine
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(metabolism, therapy)
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