[Levels of mature cross-links and advanced glycation end product cross-links in human vitreous].

Abstract

PURPOSE:

To determine the levels of pyridinoline and deoxypyridinoline, two mature enzymatic cross-links, and pentosidine, an advanced glycation end product (AGE) cross-link, in human vitreous and to investigate the correlations among the cross-links and the effects of aging and diabetes mellitus (DM) on the levels of the cross-links.

METHODS:

Forty-five vitreous samples were collected from 32 eyes undergoing vitrectomy for diabetic retinopathy (DM group) and 13 eyes (control group) from age- and sex-matched patients with idiopathic macular hole or epiretinal membrane with no systemic conditions. The levels of the cross-links were determined using high-performance liquid chromatography after acid hydrolysis and pre-treatment with SP-Sephadex.

RESULTS:

The levels of pentosidine, pyridinoline, and deoxypyridinoline were 27.3 +/- 23.1(mean +/- standrard deviation) pmol/ml (n = 45), 79.0 +/- 40.2 (mean +/- standrard deviation) ng/ml (n = 43), and 54.0 +/- 9.5 ng/ml (n = 32), respectively. When the vitreous samples from DM and the controls were compared, a significant difference (p < 0.05) was found in the level of pentosidine but not in the levels of pyridinoline or deoxypyridinoline. No significant correlations were found between age and the cross-links. Significant correlations (p < 0.01) were found among the cross-links.

CONCLUSIONS:

The results indicate that mature cross-link substances exist in human vitreous. The results also suggest that glycation may occur in the vitreous after mature cross-links form and result in the formation of AGE cross-links. In human vitreous from patients with DM, increased levels of AGE cross-links may stabilize the formation of mature cross-links but did not increase mature cross-links.

• Authors: Yukihiro Matsumoto, Masaaki Takahashi, Makoto Chikuda, Kiyomi Arai
• Journal: Nippon Ganka Gakkai zasshi (Nippon Ganka Gakkai Zasshi) Vol. 106 Issue 1 Pg. 9-15 (Jan 2002) ISSN: 0029-0203 [Print] Japan
• PMID: 11828768 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Cross-Linking Reagents
• Glycation End Products, Advanced
• pyridinoline
• deoxypyridinoline
• Arginine
• pentosidine
• Lysine

Topics

• Adult
• Aged
• Aging (physiology)
• Amino Acids (analysis)
• Arginine (analogs & derivatives, analysis)
• Cross-Linking Reagents (analysis)
• Diabetes Mellitus (metabolism)
• Glycation End Products, Advanced (analysis)
• Humans
• Lysine (analogs & derivatives, analysis)
• Middle Aged
• Vitreous Body (chemistry)