Abstract |
Expression of the alpha(v) beta6 integrin is strikingly upregulated in several types of carcinoma, including human oral squamous cell carcinoma (SCC). Employing a neutralizing monoclonal antibody to alpha(v)beta6, we investigated its role in cell adhesion, proliferation, migration, and in vivo growth of an invasive human SCC line, termed HSC-3. We found that alpha(v)beta6 is strictly required for HSC-3 cell growth in a three-dimensional collagen gel and also prominently contributes to cell migration in two different assay systems. In addition, the anti-alpha(v)beta6 antibody inhibited the invasive growth of HSC-3 cells transorally injected into nude mice. In the presence of the coinjected antibody, the average tumor size at 10 days was reduced by 59%. Histologically, antibody-treated tumors appeared less invasive than control tumors. Furthermore, intravenous application of a neutralizing antibody to the alpha(v) integrin subunit retarded HSC-3 tumor growth. These results point to a possible critical role of the alpha(v) beta6 integrin in controlling growth and invasion of human oral cancer cells.
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Authors | H Xue, A Atakilit, W Zhu, X Li, D M Ramos, R Pytela |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 288
Issue 3
Pg. 610-8
(Nov 02 2001)
ISSN: 0006-291X [Print] United States |
PMID | 11676487
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Antibodies
- Antigens, Neoplasm
- Fibronectins
- Integrins
- Receptors, Fibronectin
- integrin alphavbeta6
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Topics |
- Animals
- Antibodies
(therapeutic use)
- Antigens, Neoplasm
- Carcinogenicity Tests
- Carcinoma, Squamous Cell
(metabolism, pathology)
- Cell Adhesion
(physiology)
- Cell Division
(physiology)
- Cell Movement
(physiology)
- Disease Models, Animal
- Fibronectins
(metabolism)
- Humans
- Integrins
(immunology, physiology)
- Mice
- Mice, Nude
- Mouth Neoplasms
(metabolism, pathology)
- Neoplasm Transplantation
- Neoplasms, Experimental
(drug therapy)
- Receptors, Fibronectin
(physiology)
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
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