Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells.

Abstract

Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems.

• Authors: T Noguchi, K Ikeda, K Yamamoto, I Yoshida, A Ashiba, J Tsuchiyama, K Shinagawa, T Yoshino, M Takata, M Harada
• Journal: British journal of haematology (Br J Haematol) Vol. 114 Issue 1 Pg. 84-92 (Jul 2001) ISSN: 0007-1048 [Print] England
• PMID: 11472349 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adaptor Proteins, Signal Transducing
• Carrier Proteins
• Cytoskeletal Proteins
• Intracellular Signaling Peptides and Proteins
• LIM Domain Proteins
• Oligonucleotides, Antisense
• PDLIM7 protein, human
• Proto-Oncogene Proteins c-bcl-2

Topics

• Adaptor Proteins, Signal Transducing
• Apoptosis (drug effects)
• Blotting, Western
• Carrier Proteins (genetics)
• Cell Division (drug effects)
• Cell Line, Transformed
• Cytoskeletal Proteins
• Gene Deletion
• Gene Expression (drug effects)
• Herpesvirus 4, Human
• Humans
• Intracellular Signaling Peptides and Proteins
• Killer Cells, Natural (metabolism, virology)
• LIM Domain Proteins
• Lymphoma, T-Cell (metabolism, virology)
• Oligonucleotides, Antisense (pharmacology)
• Proto-Oncogene Proteins c-bcl-2 (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Tumor Cells, Cultured