Non-involvement of the K-ras mutation in colon carcinogenesis promoted by dietary deoxycholate in azoxymethane-treated rats.

Abstract

Fisher-344 rats, whose ileum or jejunum had been surgically removed to change the influx of bile acids into the colon, were intraperitoneally administered with azoxymethane and fed on a diet containing deoxycholate for 39 weeks to induce colon cancer. Fecal bile acids in the ileum-resected group were 1.5-times and serum bile acids were about half of those in the jejunum-resected group. As a result, the incidence and number of tumors were higher in the ileum-resected group. In the total of 59 colon tumors (40 were in the ileum-resected group and 19 in the jejunum-resected group), 56 were carcinomas, including two well-differentiated invasive and two mucinous carcinomas found in the ileum-resected rats. However, only three carcinomas, two invasive and one non-invasive, had the K-ras mutation. These results demonstrate that the K-ras mutation was not essentially involved in deoxycholate-promoted colon carcinogenesis.

• Authors: R Kanamoto, N Azuma, Y Tsuchihashi, H Suda, T Saeki, K Iwami
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 65 Issue 4 Pg. 848-52 (Apr 2001) ISSN: 0916-8451 [Print] England
• PMID: 11388463 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• Deoxycholic Acid
• Azoxymethane

Topics

• Animals
• Azoxymethane (pharmacology)
• Carcinogens (pharmacology)
• Carcinoma (chemically induced, genetics, pathology)
• Colonic Neoplasms (chemically induced, genetics, pathology)
• Deoxycholic Acid (pharmacology)
• Diet
• Genes, ras (genetics)
• Mutation (genetics)
• Polymorphism, Single-Stranded Conformational
• Rats
• Reverse Transcriptase Polymerase Chain Reaction