[CYP11B2 expression in rat liver and the efficacy of antisterone on liver fibrosis].

Abstract

OBJECTIVE:

To identify aldosterone synthase gene-CYP11B2 mRNA expression in rat livers and evaluate the efficacy of antisterone.

METHODS:

One hundred and twenty male Wistar rats were divided into 3 groups: model group (subcutaneous injection of CCl(4), 0.25 ml/100 mg, 3 times/week), antisterone group (besides same dosage of CCl(4) given, gastropufusion of antisterone of 20mg x kg(-1) x d(-1)), and control group (subcutaneous injection of olive oil). The area of collagen was examined by image analyzing system. The expression of aldosterone synthase gene-CYP11B2 mRNA was detected by RT-PCR and in situ hybridization.

RESULTS:

The expression of CYP11B2 mRNA, which located in the endoplasm of HSC, was upregulated when fibrogenesis occurred. The grade of fibrosis and the area of collagen in antisterone group were less than those in model group before 6th week (P<0.05). After the 6th week however, there was no significant difference between the two groups (P>0.05).

CONCLUSIONS:

The expression of CYP11B2 mRNA is upregulated in fibrotic liver. Antisterone can partly have a fibrogenesis-inhibiting effect on the early stage of CCl(4)-induced hepatic fibrosis.

• Authors: X Li, Y Meng, X Yang, P Wu, H Zhang, S Li, W Lai
• Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (Zhonghua Gan Zang Bing Za Zhi) Vol. 9 Issue 2 Pg. 75-7 (Apr 2001) ISSN: 1007-3418 [Print] China
• PMID: 11350681 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Cytochrome P-450 CYP11B2

Topics

• Animals
• Cytochrome P-450 CYP11B2 (genetics)
• Drugs, Chinese Herbal (therapeutic use)
• Gene Expression Regulation, Enzymologic
• Liver (cytology, enzymology)
• Liver Cirrhosis, Experimental (drug therapy, enzymology)
• Male
• Rats
• Rats, Wistar