Selective cytotoxicity of lithium gamma-linolenic acid in human T cells chronically and productively infected with HIV.

Abstract

We sought to extend observations of lithium gamma-linolenic acid (LiGLA)-associated selective cytotoxicity in different models of chronic HIV infection in vitro. In our initial experiments, 8E5, 8E5L and A3.01 cells were allowed to proliferate in the presence of 0-20 microg/ml LiGLA for 4 days. Similarly, OM-10.1 cells (with or without prior stimulation with tumour necrosis factor-alpha (TNF-alpha)) were grown with 0-5 microg/ml LiGLA for 10 days. Significant cytotoxicity was observed in productively infected 8E5 cells (100% cell death by day 2 at the highest concentration) as compared with latently infected 8E5L cells (50% cell death on days 2-4) and uninfected A3.01 cells. No drug-induced viral stimulation was observed in surviving cells. In fact, a mild direct antiviral effect may be present, independent of cytotoxicity. Maximum cytotoxicity to OM-10.1 cells was only observed when active viral replication was induced by TNF-alpha. Our preliminary results are encouraging and suggest that LiGLA should be retained as a candidate antiretroviral agent. Further work is underway to identify the specific mechanism underlying our observations.

• Authors: O Mpanju, M Winther, J Manning, K Craib, J Montaner, M O'Shaugnessy, B Conway
• Journal: Antiviral therapy (Antivir Ther) Vol. 2 Issue 1 Pg. 13-9 (Jan 1997) ISSN: 1359-6535 [Print] England
• PMID: 11322262 (Publication Type: Journal Article)

Chemical References

• Anti-HIV Agents
• Tumor Necrosis Factor-alpha
• gamma-Linolenic Acid

Topics

• Anti-HIV Agents (pharmacology)
• Cell Line
• Cell Survival (drug effects)
• HIV (drug effects)
• Humans
• Lipid Peroxidation (drug effects)
• T-Lymphocytes (drug effects, virology)
• Tumor Necrosis Factor-alpha (pharmacology)
• gamma-Linolenic Acid (pharmacology)