We have identified and cloned a human fetal lung
cDNA encoding a new
protein of the ADAM-TS family (a
disintegrin and
metalloproteinase domain, with
thrombospondin type-1 modules) that has been called
ADAM-TS12. This
protein exhibits a domain organization similar to the remaining family members including a propeptide and
metalloproteinase-like,
disintegrin-like, and
cysteine-rich domains. However, the number and organization of the TS repeats is unique with respect to other human ADAM-TSs. A total of eight
TS-1 repeats arranged in three groups are present in this novel ADAM-TS. Analysis of intracellular processing of
ADAM-TS12 revealed that it is synthesized as a precursor molecule that is first activated by cleavage of the prodomain in a
furin-mediated process and subsequently processed into two fragments of different size: a 120-kDa N-terminal proteolytically active fragment containing the
metalloproteinase and
disintegrin domains, and a 83-kDa C-terminal fragment containing most of the
TS-1 repeats. Somatic cell hybrid and radiation hybrid mapping experiments showed that the human
ADAM-TS12 gene maps to 5q35, a location that differs from all ADAM genes mapped to date. Northern blot analysis of RNAs from human adult and fetal tissues demonstrated that
ADAM-TS12 transcripts are only detected at significant levels in fetal lung but not in any other analyzed tissues. In addition,
ADAM-TS12 transcripts were detected in gastric
carcinomas and in tumor cell lines from diverse sources, being induced by
transforming growth factor-beta in KMST human fibroblasts. These data suggest that
ADAM-TS12 may play roles in pulmonary cells during fetal development or in
tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion.