Abstract | BACKGROUND: METHODS:
IL-6 secretion from MC induced by bacterial lipopolysaccharide (LPS) were assessed using sandwich ELISA. LPS-induced MC proliferation was detected by 3H-thymidine incorporation and WST-1 assay (similar to MTT assay). RESULTS: Incubation of MCs with various dosages of LPS (0, 1, 10, 50 and 100 ng/ml) induced IL-6 secretion in a dose-dependent manner. However, dilazep significantly inhibited this LPS-induced IL-6 secretion from MCs in a dose- and time-dependent manner. Dilazep also significantly inhibited MC proliferation in a dose-dependent manner. CONCLUSION:
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Authors | T Gohda, Y Makita, T Shike, K Funabiki, I Shirato, Y Tomino |
Journal | Kidney & blood pressure research
(Kidney Blood Press Res)
Vol. 24
Issue 1
Pg. 33-8
( 2001)
ISSN: 1420-4096 [Print] Switzerland |
PMID | 11174004
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-6
- Lipopolysaccharides
- Vasodilator Agents
- Dilazep
|
Topics |
- Animals
- Cell Division
(drug effects)
- Dilazep
(pharmacology)
- Drug Antagonism
- Glomerular Mesangium
(drug effects, metabolism, pathology)
- Interleukin-6
(metabolism)
- Lipopolysaccharides
(pharmacology)
- Mice
- Vasodilator Agents
(pharmacology)
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