Determination of nociceptin-orphanin FQ metabolites by capillary LC-MS.

Abstract

Nociceptin-orphanin FQ (OFQ/N) is a newly discovered peptide involved in pain transmission. The method is described to identify metabolic pathway of this neuropeptide in the spinal cord of rats using capillary size-exclusion liquid chromatography coupled to electrospray ionization mass spectrometry. The applied technique is rapid and selective, and allows for simultaneous measurement and quantitation of several fragments in the incubation mixture.

• Authors: P Suder, J Kotlińska, A Legowska, M Smoluch, G Höhne, J P Chervet, K Rolka, J Silberring
• Journal: Brain research. Brain research protocols (Brain Res Brain Res Protoc) Vol. 6 Issue 1-2 Pg. 40-6 (Nov 2000) ISSN: 1385-299X [Print] Netherlands
• PMID: 11086262 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Opioid Peptides

Topics

• Animals
• Chromatography, High Pressure Liquid
• Male
• Opioid Peptides (metabolism)
• Rats
• Rats, Wistar
• Spectrometry, Mass, Electrospray Ionization
• Spinal Cord (metabolism)
• Nociceptin