Direct association of LIS1, the lissencephaly gene product, with a mammalian homologue of a fungal nuclear distribution protein, rNUDE.

Abstract

LIS1 is a product of the causative gene for type I lissencephaly characterized by a smooth brain surface due to a defect in neuronal migration during brain development and a regulatory subunit of platelet-activating factor acetylhydrolase (PAF-AH). It is also a mammalian homologue of the fungal nuclear distribution (nud) gene, nudF, which controls the migration of fungal nuclei. Using the two-hybrid system, we identified a novel LIS1-interacting protein, rat NUDE (rNUDE), and found that it is a mammalian homologue of another fungal nud gene product, NUDE, and Xenopus mitotic phosphoprotein 43 which is phosphorylated in a cell cycle-dependent manner. rNUDE and the catalytic subunits of PAF-AH interact with the N- and C-termini of LIS1, respectively. However, these proteins, instead of simultaneously binding to LIS1, appeared to bind to LIS1 in a competitive manner. These results suggest that LIS1 functions in nuclear migration by interacting with multiple intracellular proteins in mammals.

• Authors: M Kitagawa, M Umezu, J Aoki, H Koizumi, H Arai, K Inoue
• Journal: FEBS letters (FEBS Lett) Vol. 479 Issue 1-2 Pg. 57-62 (Aug 11 2000) ISSN: 0014-5793 [Print] England
• PMID: 10940388 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Complementary
• Fungal Proteins
• GAP-43 Protein
• Microtubule-Associated Proteins
• RNA, Messenger
• RO-11 protein, Neurospora crassa
• Recombinant Proteins
• Phospholipases A
• 1-Alkyl-2-acetylglycerophosphocholine Esterase
• PAFAH1B1 protein, human

Topics

• 1-Alkyl-2-acetylglycerophosphocholine Esterase
• Amino Acid Sequence
• Animals
• Binding, Competitive
• CHO Cells
• Cell Line
• Cricetinae
• DNA, Complementary (genetics)
• Fungal Proteins (genetics, metabolism)
• GAP-43 Protein (genetics, metabolism)
• Humans
• Microtubule-Associated Proteins (genetics, metabolism)
• Molecular Sequence Data
• Phospholipases A (metabolism)
• RNA, Messenger (genetics, metabolism)
• Rats
• Recombinant Proteins (genetics, metabolism)
• Sequence Homology, Amino Acid
• Tissue Distribution
• Transfection
• Two-Hybrid System Techniques
• Xenopus