Abstract |
To investigate the molecular mechanisms of muscle atrophy under microgravity, the paraspinal muscles of rats after 14 days spaceflight and those of ground-based controls were examined. In the microgravitational environment, expressions of 42 genes changed, and the expressions of heat shock protein 70 and t complex polypeptide 1 increased. In Northern blotting, myocyte-specific enhancer binding factor 2C (MEF2C) and MEF2C-related genes including aldolase A and muscle ankyrin decreased. After 9 days ground recovery, expression of MEF2C increased and it was located mainly on the satellite cells in the muscle regeneration state. MEF2C could be a key transcriptional factor for skeletal muscle atrophy and regeneration under microgravity.
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Authors | M Yamakuchi, I Higuchi, S Masuda, Y Ohira, T Kubo, Y Kato, I Maruyama, I Kitajima |
Journal | FEBS letters
(FEBS Lett)
Vol. 477
Issue 1-2
Pg. 135-40
(Jul 14 2000)
ISSN: 0014-5793 [Print] England |
PMID | 10899324
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MEF2 Transcription Factors
- Myogenic Regulatory Factors
- RNA, Messenger
- DNA
- Myosin Heavy Chains
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Topics |
- Animals
- DNA
(genetics, metabolism)
- Down-Regulation
- Gene Expression Profiling
- MEF2 Transcription Factors
- Male
- Microscopy, Fluorescence
- Muscle Fibers, Slow-Twitch
(metabolism, pathology)
- Muscle, Skeletal
(metabolism, pathology, physiology)
- Muscular Atrophy
(genetics, metabolism)
- Myogenic Regulatory Factors
(genetics, metabolism)
- Myosin Heavy Chains
(genetics, metabolism)
- RNA, Messenger
(genetics, metabolism)
- Rats
- Rats, Sprague-Dawley
- Regeneration
- Space Flight
- Time Factors
- Weightlessness
(adverse effects)
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