MspI polymorphism at +83 bp in intron 1 of the human apolipoprotein A1 gene is associated with elevated levels of HDL cholesterol and apolipoprotein A1 in nondiabetic subjects but not in type 2 diabetic patients with coronary heart disease.

Abstract

OBJECTIVE:

Elevated HDL cholesterol and its principal carrier protein apolipoprotein a1 [apo(a1)] are associated with reduced risk of coronary heart disease (CHD). No studies are available on the impact of the -75-bp and/or +83-bp polymorphisms of the apo(a1) gene on HDL cholesterol and apo(a1) levels in patients with type 2 diabetes.

RESEARCH DESIGN AND METHODS:

We determined the prevalence of the: -75-bp and +83-bp polymorphisms of the apo(a1) gene by restriction fragment length polymorphism analysis among 308 unrelated nondiabetic subjects with CHD and among 251 unrelated patients with type 2 diabetes with CHD and in randomly selected 82 healthy men (CHD-).

RESULTS:

The rare M1- and M2- allele frequencies of the apo(a1) gene were 23 and 1.8%, respectively, among control subjects; 20 and 1.5%, respectively, among nondiabetic subjects with CHD; and 22 and 2.6%, respectively, among patients with type 2 diabetes and CHD (NS). Nonsmoking nondiabetic subjects with CHD having the M2+- genotype had higher HDL cholesterol (1.48 +/- 0.19 vs. 1.23 +/- 0.02 mmol/l, P < 0.01) and apo(a1) (1.43 +/- 0.10 vs. 1.36 +/- 0.02 g/l, P < 0.05) levels than subjects with the M2++ genotype, even after adjustment for confounding factors. This association was not found among patients with type 2 diabetes and CHD.

CONCLUSIONS:

We conclude that the +83-bp polymorphism of the apo(a1) gene is associated with elevated HDL cholesterol and apo(a1) levels in Finnish nondiabetic subjects but not in patients with type 2 diabetes.

• Authors: A Pulkkinen, L Viitanen, A Kareinen, S Lehto, M Laakso
• Journal: Diabetes care (Diabetes Care) Vol. 23 Issue 6 Pg. 791-5 (Jun 2000) ISSN: 0149-5992 [Print] United States
• PMID: 10840998 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein A-I
• Apolipoproteins B
• Blood Glucose
• Cholesterol, HDL
• Triglycerides
• Deoxyribonuclease HpaII

Topics

• Apolipoprotein A-I (blood, genetics)
• Apolipoproteins B (blood)
• Blood Glucose (metabolism)
• Blood Pressure
• Cholesterol, HDL (blood)
• Coronary Disease (blood, genetics, physiopathology)
• Deoxyribonuclease HpaII
• Diabetes Mellitus, Type 2 (blood, genetics, physiopathology)
• Diabetic Angiopathies (blood, genetics, physiopathology)
• Female
• Genotype
• Humans
• Introns
• Male
• Middle Aged
• Polymorphism, Restriction Fragment Length
• Reference Values
• Triglycerides (blood)