Copper-dependent DNA damage induced by hydrazobenzene, an azobenzene metabolite.

Abstract

Hydrazobenzene is carcinogenic to rats and mice and azobenzene is carcinogenic to rats. Hydrazobenzene is a metabolic intermediate of azobenzene. To clarify the mechanism of carcinogenesis by azobenzene and hydrazobenzene, we investigated DNA damage induced by hydrazobenzene, using 32P-5'-end-labeled DNA fragments obtained from the c-Ha-ras-1 protooncogene and the p53 tumor suppressor gene. Hydrazobenzene caused DNA damage in the presence of Cu(II). Piperidine treatment enhanced the DNA damage greatly, suggesting that hydrazobenzene caused base modification and liberation. However, azobenzene did not cause DNA damage even in the presence of Cu(II). Hydrazobenzene plus Cu(II) caused DNA damage frequently at thymine residues. Catalase and a Cu(I)-specific chelator inhibited Cu(II)-mediated DNA damage by hydrazobenzene. Typical *OH scavengers did not inhibit the DNA damage. The main active species is probably a metal oxygen complex, such as Cu(I)-OOH. Formation of 8-oxo-7, 8-dihydro-2'-deoxyguanosine was increased by hydrazobenzene in the presence of Cu(II). Oxygen consumption and UV-Visible spectroscopic measurements have shown that hydrazobenzene is autoxidized to azobenzene with H2O2 formation. It is considered that the metal-mediated DNA damage by hydrazobenzene through H2O2 generation may be relevant for the expression of carcinogenicity of azobenzene and hydrazobenzene.

• Authors: S Ohnishi, M Murata, S Oikawa, Y Hiraku, S Kawanishi
• Journal: Free radical research (Free Radic Res) Vol. 32 Issue 6 Pg. 469-78 (Jun 2000) ISSN: 1071-5762 [Print] England
• PMID: 10798712 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Azo Compounds
• Chelating Agents
• Free Radical Scavengers
• Phenanthrolines
• Phenylhydrazines
• Piperidines
• 1,2-diphenylhydrazine
• Hydroxyl Radical
• piperidine
• Copper
• 8-Hydroxy-2'-Deoxyguanosine
• DNA
• bathocuproine
• Hydrogen Peroxide
• Catalase
• azobenzene
• Deoxyguanosine

Topics

• 8-Hydroxy-2'-Deoxyguanosine
• Animals
• Azo Compounds (metabolism)
• Catalase (pharmacology)
• Cattle
• Chelating Agents (pharmacology)
• Copper (pharmacology)
• DNA (metabolism)
• DNA Damage
• Deoxyguanosine (analogs & derivatives, metabolism)
• Free Radical Scavengers
• Humans
• Hydrogen Peroxide (metabolism)
• Hydroxyl Radical (metabolism)
• Oxidation-Reduction
• Phenanthrolines (pharmacology)
• Phenylhydrazines (pharmacology)
• Piperidines (pharmacology)