Molecular evidence of simian virus 40 infections in children.

Abstract

Recent studies have detected simian virus 40 (SV40) DNA in certain human tumors and normal tissues. The significance of human infections by SV40, which was first discovered as a contaminant of poliovirus vaccines used between 1955 and 1963, remains unknown. The occurrence of SV40 infections in unselected hospitalized children was evaluated. Polymerase chain reaction and DNA sequence analyses were done on archival tissue specimens from patients positive for SV40 neutralizing antibody. SV40 DNA was identified in samples from 4 of 20 children (1 Wilms' tumor, 3 transplanted kidney samples). Sequence variation among SV40 regulatory regions ruled out laboratory contamination of specimens. This study shows the presence of SV40 infections in pediatric patients born after 1982.

• Authors: J S Butel, A S Arrington, C Wong, J A Lednicky, M J Finegold
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 180 Issue 3 Pg. 884-7 (Sep 1999) ISSN: 0022-1899 [Print] United States
• PMID: 10438386 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Viral
• DNA, Viral

Topics

• Antibodies, Viral (blood)
• Child
• DNA, Viral (analysis)
• Humans
• Kidney Neoplasms (blood, pathology, virology)
• Kidney Transplantation
• Papillomavirus Infections (blood, diagnosis, pathology)
• Polymerase Chain Reaction
• Retrospective Studies
• Simian virus 40 (genetics, isolation & purification)
• Tumor Virus Infections (blood, diagnosis, pathology)
• Wilms Tumor (blood, pathology, virology)