Effects of XT-44, a phosphodiesterase 4 inhibitor, in osteoblastgenesis and osteoclastgenesis in culture and its therapeutic effects in rat osteopenia models.

Abstract

We have reported that denbufylline, a phosphodiesterase 4 (PDE4) inhibitor, inhibits bone loss in Walker256/S tumor-bearing rats, suggesting therapeutic potentiality of a PDE4 inhibitor in osteopenia. In the present study, effects of a new PDE4 inhibitor, 1-n-butyl-3-n-propylxanthine (XT-44), in bone were evaluated in cell cultures and animal experiments. In rat bone marrow culture, XT-44 stimulated mineralized-nodule formation, whereas it inhibited osteoclast-like cell formation in mouse bone marrow culture. In Walker256/S-bearing rats (6-week-old female Wistar Imamichi rats), rapid decrease in bone mineral density (BMD) was prominent, and oral administration of XT-44 (0.3 mg/kg, every 2 days) inhibited the decrease in BMD. In the second animal experiment, female Wistar rats (6-week-old) were sciatic neurectomized, and XT-44 was orally administered to these rats every 2 days for 4 weeks. XT-44 administration (0.3 mg/kg) recovered BMD in these neurectomized animals. Furthermore, 19-week-old, female Wistar rats were ovariectomized (OVX), and 15 weeks after surgery, these rats were orally administered XT-44 every 2 days for 8 weeks. XT-44 treatment (1 mg/kg) increased the BMD of OVX rats. These results indicate that XT-44 could be a candidate as a therapeutic drug for treating osteopenia including osteoporosis.

• Authors: Y Waki, T Horita, K Miyamoto, K Ohya, S Kasugai
• Journal: Japanese journal of pharmacology (Jpn J Pharmacol) Vol. 79 Issue 4 Pg. 477-83 (Apr 1999) ISSN: 0021-5198 [Print] Japan
• PMID: 10361888 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 1-n-butyl-3-n-propylxanthine
• Phosphodiesterase Inhibitors
• Purinones
• Pyrrolidinones
• Xanthines
• 3',5'-Cyclic-AMP Phosphodiesterases
• Cyclic Nucleotide Phosphodiesterases, Type 4
• zaprinast
• Rolipram
• 1-Methyl-3-isobutylxanthine

Topics

• 1-Methyl-3-isobutylxanthine (pharmacology)
• 3',5'-Cyclic-AMP Phosphodiesterases (antagonists & inhibitors)
• Animals
• Body Weight (drug effects)
• Bone Density (drug effects)
• Bone Diseases, Metabolic (drug therapy)
• Bone Marrow Cells (cytology, drug effects)
• Cyclic Nucleotide Phosphodiesterases, Type 4
• Denervation
• Dose-Response Relationship, Drug
• Female
• Femur (metabolism)
• Giant Cells (cytology, drug effects)
• Male
• Mice
• Osteoblasts (cytology, drug effects)
• Osteoclasts (cytology, drug effects)
• Osteogenesis (drug effects)
• Ovariectomy
• Phosphodiesterase Inhibitors (pharmacology)
• Purinones (pharmacology)
• Pyrrolidinones (pharmacology)
• Rats
• Rats, Wistar
• Rolipram
• Sciatic Nerve (surgery)
• Xanthines (pharmacology)