The effect of thrombospondin on oral squamous carcinoma cell invasion of collagen.

Abstract

BACKGROUND:

Thrombospondin (TSP), a cell matrix protein, and transforming growth factor beta (TGF-beta), a growth regulatory protein, play roles in tumor progression. The purpose of this study was to investigate the effects of TSP and TGF-beta on tumor cell invasion.

MATERIALS AND METHODS:

Tumor cell invasion assays were performed using a modified Boyden chamber apparatus with collagen-coated membranes. The KB oral carcinoma cell line was studied in serum-free media. Invasion was measured as the summation of the number of cells in five representative low-power fields (x 100) traversing the collagen barrier after a 3-hour incubation period. The effects of antibodies against TSP, TGF-beta and the cysteine-serine-valine-threonine-cysteine-glycine (CSVTCG)-specific TSP receptor were also evaluated.

RESULTS:

TSP caused a dose-dependent stimulation of tumor cell invasion. Antibodies against TSP, its CSVTCG-specific receptor, and TGF-beta inhibited TSP-promoted invasion by 50% to 71%.

CONCLUSIONS:

TSP and its CSVTCG-specific receptor promote KB cell invasion of collagen through the production and/or activation of TGF-beta.

• Authors: T N Wang, X H Qian, M S Granick, M P Solomon, V L Rothman, G P Tuszynski
• Journal: American journal of surgery (Am J Surg) Vol. 170 Issue 5 Pg. 502-5 (Nov 1995) ISSN: 0002-9610 [Print] United States
• PMID: 7485743 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• CD36 Antigens
• Cell Adhesion Molecules
• Culture Media, Serum-Free
• Immunoglobulin G
• Integrins
• Membrane Glycoproteins
• Membranes, Artificial
• Polycarboxylate Cement
• Thrombospondins
• Transforming Growth Factor beta
• polycarbonate
• Collagen

Topics

• Amino Acid Sequence
• Antibodies, Monoclonal
• CD36 Antigens (drug effects, immunology, physiology)
• Carcinoma, Squamous Cell (metabolism, pathology)
• Cell Adhesion Molecules (administration & dosage, immunology, pharmacology)
• Cell Count
• Collagen (drug effects, metabolism)
• Culture Media, Serum-Free
• Diffusion Chambers, Culture
• Disease Progression
• Dose-Response Relationship, Drug
• Humans
• Immunoglobulin G
• Integrins (drug effects, immunology, physiology)
• Membrane Glycoproteins (administration & dosage, immunology, pharmacology)
• Membranes, Artificial
• Microscopy, Phase-Contrast
• Mouth Neoplasms (metabolism, pathology)
• Neoplasm Invasiveness
• Polycarboxylate Cement
• Thrombospondins
• Transforming Growth Factor beta (immunology, pharmacology)
• Tumor Cells, Cultured