Polygonum perfoliatum L. ethanol extract ameliorates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin inflammation.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Polygonum perfoliatum L. (PP) is classified as a heat-clearing and detoxifying agent in traditional Chinese medicine, and is believed to possess therapeutic properties for treating eczema, furuncles, and venomous snake bites. Previous studies have demonstrated that PP extract exhibits multiple bioactivities, including antibacterial, anti-inflammatory, antitumor, antioxidation, and antiviral properties. However, no existing studies have evaluated the effects of PP on animal models of atopic dermatitis (AD)-like skin symptoms, which are closely associated with traditional ethnic usage.

AIM OF THE STUDY:

In present study, therefore, we aimed to explore the potential anti-atopic effect of Polygonum perfoliatum L. ethanol extract (PPE) in 2,4-Dinitrochlorobenzene (DNCB)-induced dermatitis-like skin lesions.

MATERIALS AND METHODS:

For reaching this aim, DNCB-induced mice with AD-like skin inflammation were subjected to topical administration of PPE gels for a period of 21 days, and subsequently, the biological impacts of PPE were evaluated.

RESULTS:

PPE gels effectively mitigated AD-like skin symptoms induced by DNCB in mice, as demonstrated by a marked reduction in epidermal thickness and dermatitis severity. Moreover, PPE significantly decreased the production of various cytokines, including TNF-α, IL-6, IL-1β, IL-4, IL-5, IL-13 and IgE, in addition to suppressed the production of key inflammation-related enzymes (iNOS and COX-2) and decreased the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB in AD-like skin samples. Furthermore, PPE treatment inhibited the abnormally elevated CD4+/CD8+ ratio in DNCB-induced AD mice. The results of the skin irritation test revealed that PPE exhibited no adverse toxicity in mice at dose of 10 mg/day.

CONCLUSIONS:

PPE exhibits potential as a safe therapeutic agent for atopic dermatitis by efficiently mitigating DNCB-induced atopic symptoms and diminishing inflammation, and does not carry the risk of over-immunosuppression or treatment-associated adverse effects.

• Authors: Pinglong Fan, Shuchun Xie, ZhiQian Zhang, Quan Yuan, Jiajiang He, Jie Zhang, Xinyue Liu, Xiaoyi Liu, Lieqiang Xu
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 319 Issue Pt 2 Pg. 117288 (Jan 30 2024) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 37827300 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Elsevier B.V. All rights reserved.

Chemical References

• Dinitrochlorobenzene
• Ethanol
• Cytokines
• NF-kappa B
• Gels

Topics

• Animals
• Mice
• Dermatitis, Atopic (chemically induced, drug therapy, metabolism)
• Dinitrochlorobenzene
• Skin (pathology)
• Polygonum
• Ethanol (pharmacology)
• Cytokines (metabolism)
• Inflammation (drug therapy)
• Skin Diseases (metabolism)
• NF-kappa B (metabolism)
• Gels (pharmacology)
• Mice, Inbred BALB C