Imbalance of alveolar epithelial type I and type Ⅱ cells in lipopolysaccharide-induced chronic lung injury young mouse model.

Abstract

Children are susceptible to pneumonia, which affects their growth and development. Immune disorders and unrepaired alveolar mucosal epithelium following pneumonia cause chronic lung injury. The mechanism of chronic lung injury is unknown and lacks animal models for reference. Therefore, we developed a chronic lung injury young mouse model to simulate the pathological process of children. 3-week-old mice were intratracheal instillation of lipopolysaccharide (LPS) every other day for six weeks. Consequently, the histopathology showed damaged integrity of lung tissue, fibrosis, and abnormally distributed alveolar epithelial cells. The total protein concentration in bronchoalveolar lavage fluid (BALF) was increased, alveolar epithelial type (AT) I cells were abnormal distribution, and AT II cells were reduced. The phosphorylation levels of IKBα and the expression levels of NF-κB p65 in lung tissue were up-regulated. In serum and BALF, the IL-6 was oversecretion, nitric oxide (NO) and superoxide dismutase (SOD) were perturbed secretion, oxidative stress imbalance. In addition, blood viscosity, plasma viscosity, and erythrocyte sedimentation rate (ESR) indexes in hemorheology were increased. In conclusion, it is feasible to construct the mouse model of chronic lung injury, and AT I and AT Ⅱ cells were imbalanced, which paves the way for further investigations on the pathogenesis of chronic lung injury and the efficacy of novel treatments.

• Authors: Jianning Guo, Hongxian Zhang, Xue Bai, Junming Liang, Ziyi Guo, Yan Liu, Ning Ma, Handong Wang, Ruijuan Dong, Xue Yu, Dongyu Ge, Xia Cui
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 618 Pg. 107-112 (08 27 2022) ISSN: 1090-2104 [Electronic] United States
• PMID: 35716594 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2022 Elsevier Inc. All rights reserved.

Chemical References

• Lipopolysaccharides
• NF-kappa B

Topics

• Animals
• Bronchoalveolar Lavage Fluid
• Disease Models, Animal
• Lipopolysaccharides (metabolism)
• Lung (pathology)
• Lung Injury (pathology)
• Mice
• NF-kappa B (metabolism)
• Pneumonia (chemically induced)