To determine whether pro-inflammatory
lipid lysophosphatidylinositols (LPIs) upregulate the expressions of
membrane proteins for adhesion/signaling and secretory
proteins in human aortic endothelial cell (HAEC) activation, we developed an EC biology knowledge-based transcriptomic formula to profile
RNA-Seq data panoramically. We made the following primary findings: first,
G protein-coupled receptor 55 (GPR55), the LPI receptor, is expressed in the endothelium of both human and mouse aortas, and is significantly upregulated in
hyperlipidemia; second, LPIs upregulate 43 clusters of differentiation (CD) in HAECs, promoting EC activation, innate immune trans-differentiation, and immune/inflammatory responses; 72.1% of LPI-upregulated CDs are not induced in influenza virus-, MERS-CoV virus- and herpes virus-infected human endothelial cells, which hinted the specificity of LPIs in HAEC activation; third, LPIs upregulate six types of 640 secretomic genes (SGs), namely, 216 canonical SGs, 60 caspase-1-gasdermin D (GSDMD) SGs, 117
caspase-4/11-GSDMD SGs, 40 exosome SGs, 179 Human
Protein Atlas (HPA)-
cytokines, and 28 HPA-
chemokines, which make HAECs a large secretory organ for
inflammation/immune responses and other functions; fourth, LPIs activate transcriptomic remodeling by upregulating 172
transcription factors (TFs), namely, pro-inflammatory factors NR4A3, FOS, KLF3, and HIF1A; fifth, LPIs upregulate 152 nuclear
DNA-encoded mitochondrial (mitoCarta) genes, which alter mitochondrial mechanisms and functions, such as mitochondrial organization, respiration, translation, and transport; sixth, LPIs activate
reactive oxygen species (ROS) mechanism by upregulating 18 ROS regulators; finally, utilizing the Cytoscape software, we found that three mechanisms, namely, LPI-upregulated TFs, mitoCarta genes, and ROS regulators, are integrated to promote HAEC activation. Our results provide novel insights into aortic EC activation, formulate an EC biology knowledge-based transcriptomic profile strategy, and identify new targets for the development of
therapeutics for
cardiovascular diseases, inflammatory conditions,
immune diseases,
organ transplantation, aging, and
cancers.