Long non-coding RNA MALAT1 regulates cell proliferation and apoptosis via miR-135b-5p/GPNMB axis in Parkinson's disease cell model.

Abstract

BACKGROUNDS:

Parkinson's disease (PD) is a common age-related neurodegenerative disorder worldwide. This research aimed to investigate the effects and mechanism underlying long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in PD.

METHODS:

SK-N-SH and SK-N-BE cells were treated with MPP+ to establish the MPP+-stimulated cell model of PD, and MALAT1 expression was determined. Then, the effects of MALAT1 depletion on cell proliferation and apoptosis were determined in the MPP+-stimulated cell model of PD. Besides, the correlations between microRNA-135b-5p (miR-135b-5p) and MALAT1 or glycoprotein nonmetastatic melanoma protein B (GPNMB) in MPP+-stimulated cell model of PD were explored.

RESULTS:

MALAT1 was increasingly expressed and downregulation of MALAT1 promoted cell proliferation while inhibited apoptosis in MPP+-stimulated cells. Besides, miR-135b-5p was a target of MALAT1 and directly targeted to GPNMB. Further investigation indicated that suppression of MALAT1 regulated cell proliferation and apoptosis by miR-135b-5p/GPNMB axis.

CONCLUSION:

Our findings reveal that MALAT1/miR-135b-5p/GPNMB axis regulated cell proliferation and apoptosis in MPP+-stimulated cell model of PD, providing a potential biomarker and therapeutic target for PD.

• Authors: Kefeng Lv, Yuhua Liu, Yanbing Zheng, Shaowen Dai, Peifeng Yin, Haifeng Miao
• Journal: Biological research (Biol Res) Vol. 54 Issue 1 Pg. 10 (Mar 16 2021) ISSN: 0717-6287 [Electronic] England
• PMID: 33726823 (Publication Type: Journal Article)

Chemical References

• GPNMB protein, human
• MALAT1 long non-coding RNA, human
• MIRN135 microRNA, human
• Membrane Glycoproteins
• MicroRNAs
• RNA, Long Noncoding

Topics

• Apoptosis
• Cell Proliferation
• Cells, Cultured
• Humans
• Membrane Glycoproteins (genetics)
• MicroRNAs (genetics)
• Parkinson Disease (genetics)
• RNA, Long Noncoding (genetics)