Transcriptomic Analysis of Stem Cells Treated with Moringin or Cannabidiol: Analogies and Differences in Inflammation Pathways.

Abstract

Inflammation is a common feature of many neurodegenerative diseases. The treatment of stem cells as a therapeutic approach to repair damage in the central nervous system represents a valid alternative. In this study, using Next-Generation Sequencing (NGS) technology, we analyzed the transcriptomic profile of human Gingival Mesenchymal Stem Cells (hGMSCs) treated with Moringin [4-(α-l-ramanosyloxy)-benzyl isothiocyanate] (hGMSCs-MOR) or with Cannabidiol (hGMSCs-CBD) at dose of 0.5 or 5 µM, respectively. Moreover, we compared their transcriptomic profiles in order to evaluate analogies and differences in pro- and anti-inflammatory pathways. The hGMSCs-MOR selectively downregulate TNF-α signaling from the beginning, reducing the expression of TNF-α receptor while hGMSCs-CBD limit its activity after the process started. The treatment with CBD downregulates the pro-inflammatory pathway mediated by the IL-1 family, including its receptor while MOR is less efficient. Furthermore, both the treatments are efficient in the IL-6 signaling. In particular, CBD reduces the effect of the pro-inflammatory JAK/STAT pathway while MOR enhances the pro-survival PI3K/AKT/mTOR. In addition, both hGMSCs-MOR and hGMSCs-CBD improve the anti-inflammatory activity enhancing the TGF-β pathway.

• Authors: Luigi Chiricosta, Serena Silvestro, Jacopo Pizzicannella, Francesca Diomede, Placido Bramanti, Oriana Trubiani, Emanuela Mazzon
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 20 Issue 23 (Nov 30 2019) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 31801206 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• IL6 protein, human
• Interleukin-1
• Interleukin-6
• Isothiocyanates
• Receptors, Interleukin-1
• STAT Transcription Factors
• Transforming Growth Factor beta
• Tumor Necrosis Factor-alpha
• moringin
• Cannabidiol
• MTOR protein, human
• Janus Kinases
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases

Topics

• Anti-Inflammatory Agents (pharmacology)
• Cannabidiol (pharmacology)
• Gene Expression Profiling
• Gene Expression Regulation
• Gingiva (cytology, drug effects, immunology)
• Humans
• Interleukin-1 (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Isothiocyanates (pharmacology)
• Janus Kinases (genetics, immunology)
• Mesenchymal Stem Cells (cytology, drug effects, immunology)
• Phosphatidylinositol 3-Kinases (genetics, immunology)
• Primary Cell Culture
• Proto-Oncogene Proteins c-akt (genetics, immunology)
• Receptors, Interleukin-1 (genetics, immunology)
• STAT Transcription Factors (genetics, immunology)
• Signal Transduction (drug effects, genetics, immunology)
• TOR Serine-Threonine Kinases (genetics, immunology)
• Transcriptome (drug effects, immunology)
• Transforming Growth Factor beta (genetics, immunology)
• Tumor Necrosis Factor-alpha (genetics, immunology)