Abstract | BACKGROUND:
Mucin 13 (MUC13) is a cell surface glycoprotein aberrantly expressed in a variety of epithelial carcinomas. Thus far, the role of MUC13 in various diseases remains elusive. To the best of our knowledge, this is the first study to examine the potential of MUC13 as a serum biomarker in a variety of carcinomas and other conditions. METHODS: We developed a recombinant MUC13 protein, mouse monoclonal antibodies and enzyme immunoassay (ELISA) for MUC13. We used this assay to measure MUC13 levels in the supernatants of cancer cell lines and a large cohort of serum samples from healthy and diseased individuals. RESULTS: CONCLUSIONS: Serum MUC13 is frequently elevated not only in a variety of malignant cases but also in some benign pathologies, thus appearing to be a non-specific disease biomarker. Nonetheless, serum MUC13 is clearly highly elevated in some carcinoma patients, and its relationship with tumor progression in this context warrant further research. Future studies that examine the correlation between serum MUC13 levels to stage of cancer could elucidate prognostic potential.
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Authors | Panagiota S Filippou, Annie H Ren, Dimitrios Korbakis, Lampros Dimitrakopoulos, Antoninus Soosaipillai, Vivian Barak, Shahar Frenkel, Jacob Pe'er, Michal Lotem, Sharon Merims, Rafael Molina, Ivan Blasutig, Dimitrios P Bogdanos, Eleftherios P Diamandis |
Journal | Clinical chemistry and laboratory medicine
(Clin Chem Lab Med)
Vol. 56
Issue 11
Pg. 1945-1953
(10 25 2018)
ISSN: 1437-4331 [Electronic] Germany |
PMID | 29768245
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- MUC13 protein, human
- Mucins
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Topics |
- Autoimmune Diseases
(diagnosis, metabolism)
- Biomarkers, Tumor
(analysis, blood)
- Carcinoma
(diagnosis, metabolism)
- Cell Line, Tumor
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Humans
- Male
- Melanoma
(diagnosis, metabolism)
- Mucins
(analysis, blood)
- Ovarian Neoplasms
(diagnosis, metabolism)
- Skin Neoplasms
(diagnosis, metabolism)
- Melanoma, Cutaneous Malignant
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