Intestinal
ischemia reperfusion (I/R) may cause
inflammation-, oxidative stress-, and apoptosis-related tissue
injuries and facilitate
bacterial infection, leading to
multiple organ failure.
Myricetin, a
flavonoid, is found to have diverse biological effects including anti-inflammatory, anti-oxidative, and anti-bacterial effects. Based on our pre-experiment, we proposed that
myricetin pretreatment (25, 50mg/kg) could ameliorate intestinal I/R injury and
myricetin-induced modulation on MKK7/JNK signal pathway might play a key role in the amelioration. The present study was designed to verify the proposal by using both rat intestinal I/R model in vivo and
hypoxia/reoxygenation (H/R)-injured intestinal epithelial cell line (IEC-6 cells) model in vitro. The results confirmed our proposal.
Myricetin selectively ameliorated I/R- and H/R-induced
injuries in vivo and in vitro respectively without significantly affecting the corresponding normal controls.
Myricetin significantly alleviated I/R-induced rat intestinal injury by reducing the generation of pro-inflammatory
cytokines including TNF-α, IL-1β, and
IL-6 and by reducing MPO activity.
Myricetin significantly reduced oxidative stress through decreasing MDA level and increasing the levels of SOD and GSH in the intestinal tissues compared with I/R control rats.
Myricetin significantly decreased apoptosis by selectively down-regulating the expression of p-MKK7 and p-JNK without affecting MKK7 and JNK, inhibiting Bax,
caspase-3 protein expression, and up-regulating Bcl-2
protein expression in I/R-injured jejunum of rats. In vitro study indicated that MKK7
siRNA transfection significantly decreased both MKK7 and p-MKK7 and other apoptosis-related
proteins, partially simulating
myricetin-induced anti-apoptotic effects. MKK7
siRNA transfection+myricetin could not further decrease MKK7, p-MKK7, and other apoptosis-related
proteins, suggesting that inhibition of MKK7/JNK pathway plays a key role in
myricetin-induced protection against intestinal I/R. MKK7 overexpression by
cDNA transfection abrogated
myricetin-reduced apoptosis-related
protein expression, confirming that the MKK7/JNK signal pathway is the key target for
myricetin-induced amelioration. The present study indicated that pretreatment of
myricetin induced selective protection against intestinal I/R injury without significantly affecting corresponding normal controls and p-MKK7 was the key target, suggesting that
myricetin is worth further translational studies.