Cell Penetrating Peptide Derived from Human Eosinophil Cationic Protein Decreases Airway Allergic Inflammation.

Abstract

Cell penetrating peptide derived from human eosinophil cationic protein (CPPecp) is a 10-amino-acid peptide containing a core heparan sulfate (HS)-binding motif of human eosinophil cationic protein (ECP). It binds and penetrates bronchial epithelial cells without cytotoxic effects. Here we investigated airway-protective effects of CPPecp in BEAS-2B cell line and mite-induced airway allergic inflammation in BALB/c mice. In BEAS-2B cell, CPPecp decreases ECP-induced eotaxin mRNA expression. CPPecp also decreases eotaxin secretion and p-STAT6 activation induced by ECP, as well as by IL-4. In vivo studies showed CPPecp decreased mite-induced airway inflammation in terms of eosinophil and neutrophil count in broncho-alveolar lavage fluid, peri-bronchiolar and alveolar pathology scores, cytokine production in lung protein extract including interleukin (IL)-5, IL-13, IL-17A/F, eotaxin; and pause enhancement from methacholine stimulation. CPPecp treated groups also showed lower serum mite-specific IgE level. In this study, we have demonstrated the in vitro and in vivo anti-asthma effects of CPPecp.

• Authors: Lin-Shien Fu, Yu-Rou Wu, Shun-Lung Fang, Jaw-Ji Tsai, Heng-Kuei Lin, Yee-Jun Chen, Ting-Yu Chen, Margaret Dah-Tsyr Chang
• Journal: Scientific reports (Sci Rep) Vol. 7 Issue 1 Pg. 12352 (09 27 2017) ISSN: 2045-2322 [Electronic] England
• PMID: 28955044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Anti-Asthmatic Agents
• Antigens, Dermatophagoides
• Cell-Penetrating Peptides
• Cytokines
• Recombinant Proteins
• Eosinophil Cationic Protein
• RNASE3 protein, human

Topics

• Allergens (immunology)
• Animals
• Anti-Asthmatic Agents (pharmacology, therapeutic use)
• Antigens, Dermatophagoides (immunology)
• Asthma (drug therapy, immunology, pathology)
• Bronchi (cytology, drug effects, immunology)
• Cell Line
• Cell-Penetrating Peptides (chemistry, pharmacology, therapeutic use)
• Cytokines (immunology, metabolism)
• Drug Evaluation, Preclinical
• Eosinophil Cationic Protein (chemistry)
• Eosinophils (immunology)
• Epithelial Cells (drug effects, immunology)
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• Neutrophils (immunology)
• Recombinant Proteins (chemistry, pharmacology, therapeutic use)
• Respiratory Mucosa (cytology, drug effects, immunology)
• Treatment Outcome