Biochanin-A induces apoptosis and suppresses migration in FaDu human pharynx squamous carcinoma cells.

Abstract

The aim of the present study was to investigate biochanin-A-induced anticancer effects and their cellular signaling pathway in FaDu pharyngeal squamous carcinoma cells. Biochanin-A induced cell death through increased cytotoxicity of FaDu cells in a dose- and time-dependent manner. The number of cells with nucleus condensation and the apoptotic population were increased in the FaDu cells stimulated with biochanin-A for 24 h. Furthermore, extrinsic apoptotic factors such as FasL and their downstream target caspase-8 were increased and activated in the FaDu cells treated with biochanin-A in a dose-dependent manner. Moreover, biochanin-A decreased the expression of intrinsic anti-apoptotic factors such as Bcl-2 and Bcl-xL, and increased the level and activation of intrinsic apoptotic factors such as Bad and caspase-9. Finally, biochanin-A induced the activation of caspase-3 and Poly(ADP ribose) polymerase (PARP) in FaDu cells. Our results suggest that biochanin-A-induced apoptosis was mediated by death receptor mediated-extrinsic and mitochondria-dependent intrinsic apoptotic signaling pathways. Biochanin-A also inhibited wound healing migration and proliferation of FaDu cells via the downregulation and inactivation of matrix metalloproteinase-2 and -9 that are mediated by the suppression of p38, mitogen activated protein kinase (MAPK), NF-κB and Akt cellular signaling pathways. Therefore, these data suggest that the biochanin-A may act as a potential chemotherapeutic compound to treat head and neck cancer.

• Authors: In-A Cho, Sang-Joun You, Kyeong-Rok Kang, Su-Gwan Kim, Ji-Su Oh, Jae-Seek You, Gyeong-Je Lee, Yo-Seob Seo, Do Kyung Kim, Chun Sung Kim, Sook-Young Lee, Jae-Sung Kim
• Journal: Oncology reports (Oncol Rep) Vol. 38 Issue 5 Pg. 2985-2992 (Nov 2017) ISSN: 1791-2431 [Electronic] Greece
• PMID: 28901495 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Apoptosis Regulatory Proteins
• Genistein
• biochanin A

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis
• Apoptosis Regulatory Proteins (metabolism)
• Carcinoma, Squamous Cell (drug therapy, metabolism)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Gene Expression Regulation, Neoplastic (drug effects)
• Gene Regulatory Networks (drug effects)
• Genistein (pharmacology)
• Humans
• Pharyngeal Neoplasms (drug therapy, metabolism)
• Time Factors