Abstract | BACKGROUND: METHODS: A clinically relevant two-hit mouse model of VILI, with intravenous lipopolysaccharide (LPS, 20 ng/mouse) immediately before high tidal volume (HTV, 20 mL/kg) ventilation (LPS+HTV), was established. Blood gas and respiratory mechanics were measured to ensure the development of VILI. Flow cytometry and histopathological analyses revealed pulmonary infiltration of leukocytes subsets. Clodronate liposomes were intravenously injected to deplete pulmonary monocytes. In vitro endothelial cell permeability assay with sorted Ly6C+high monocytes condition media assessed the role of Ly6C+high monocytes in vascular permeability. RESULTS: LPS+HTV significantly increased total proteins, TNF-α, IL-6, vascular endothelial growth factor ( VEGF) and mononuclear cells in the bronchoalveolar lavage fluid (BALF). Pulmonary Ly6C+high monocytes (SSClowCD11b+F4/80+Ly6C+high), but not Ly6C+low monocytes (SSClowCD11b+F4/80+Ly6C+low), were significantly elevated starting at 4 hr. Clodronate liposomes were able to significantly reduce pulmonary Ly6C+high monocytes, and VEGF and total protein in BALF, and restore PaO2/FiO2. There was a strong correlation between pulmonary Ly6C+high monocytes and BALF VEGF (R2 = 0.8791, p<0.001). Moreover, sorted Ly6C+high monocytes were able to produce VEGF, resulting in an increased permeability of endothelial cell monolayer in an in vitro endothelial cell permeability assay. CONCLUSION:
VEGF produced by pulmonary infiltrated Ly6C+high monocytes regulates vasculature permeability in a two-hit model of HTV-induced lung injury. Ly6C+high monocytes play an important role in the pathogenesis of VILI.
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Authors | Chung-Sheng Shi, Tzu-Hsiung Huang, Chin-Kuo Lin, Jhy-Ming Li, Mei-Hsin Chen, Mei-Ling Tsai, Chih-Ching Chang |
Journal | PloS one
(PLoS One)
Vol. 11
Issue 10
Pg. e0165317
( 2016)
ISSN: 1932-6203 [Electronic] United States |
PMID | 27783650
(Publication Type: Journal Article)
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Chemical References |
- Antigens, Ly
- Interleukin-6
- Lipopolysaccharides
- Ly-6C antigen, mouse
- Tumor Necrosis Factor-alpha
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Antigens, Ly
(metabolism)
- Blood Gas Analysis
- Bronchoalveolar Lavage Fluid
(chemistry, cytology)
- Cell Membrane Permeability
(drug effects)
- Disease Models, Animal
- Endothelial Cells
(cytology, metabolism)
- Interleukin-6
(metabolism)
- Lipopolysaccharides
(toxicity)
- Lung
(pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Monocytes
(cytology, drug effects, metabolism)
- Neutrophils
(cytology, drug effects, metabolism)
- Regression Analysis
- Respiration, Artificial
(adverse effects)
- Severity of Illness Index
- Tumor Necrosis Factor-alpha
(metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
- Ventilator-Induced Lung Injury
(metabolism, pathology)
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