C-Reactive Protein and Inflammatory Cytokines during Percutaneous Coronary Intervention.

Abstract

BACKGROUND:

C-reactive protein (CRP) is significantly associated with cardiovascular diseases; however, whether CRP plays a causal role in coronary artery disease has yet to be determined. In addition, the relationship between CRP, atherosclerosis, and inflammation remains controversial.

METHODS AND RESULTS:

Serum interleukin (IL)-6, IL-1β, and CRP levels were determined in 160 patients at time points around percutaneous coronary intervention (PCI) with drug-eluting stent implantation. The levels were found to be at peak at 24 h post-PCI and gradually declined to the level before PCI at day 30 post-PCI. These inflammation markers around PCI have no statistical difference in the different postdilation pressures (≤14, 14-18, and ≥18 atm) and stent number (1 and ≥2 stents) groups. Treatment of cultured human vascular smooth muscle cells (VSMCs) with a combination of IL-6 and IL-1β at concentrations associated with PCI did not result in any significant change in the CRP mRNA levels. The IL-6-augmented CRP expression in human internal mammary arteries (IMAs) stretched with a mechanical strength of 3 g was blocked by the nuclear factor-κB (NF-κB) peptide inhibitor SN50 and not by the inactive SN50 analog SN50M. IL-6 treatment increased NF-κB activity in human IMAs stretched with 3 g, and this effect was further blocked by stretch-activated channel (SAC) inhibitors (streptomycin or GdCl3) and SN50.

CONCLUSIONS:

The current study provides evidence that increased serum IL-6, IL-1β, and CRP levels around PCI are not different between different postdilation pressure and stent number groups. The combination of IL-6 and IL-1β at concentrations associated with PCI cannot induce CRP expression in human VSMCs, but they can augment mechanical strain-induced CRP synthesis via the SAC-NF-κB pathway in human IMAs.

• Authors: Mingxin Wu, Xinyuan Gu, Xiujuan Li, Yanhui Li, Huaineng Zhou, Guihua Lu, ZhongKai Wu, He Huang, Lilong Tang, Jianping Zeng
• Journal: Journal of vascular research (J Vasc Res) Vol. 53 Issue 1-2 Pg. 39-48 ( 2016) ISSN: 1423-0135 [Electronic] Switzerland
• PMID: 27487299 (Publication Type: Journal Article)
• Copyright: © 2016 S. Karger AG, Basel.

Chemical References

• IL1B protein, human
• IL6 protein, human
• Inflammation Mediators
• Interleukin-1beta
• Interleukin-6
• Ion Channels
• NF-kappa B
• C-Reactive Protein

Topics

• Aged
• Angioplasty, Balloon, Coronary (instrumentation)
• C-Reactive Protein (genetics, metabolism)
• Cells, Cultured
• Coronary Artery Disease (blood, diagnostic imaging, genetics, therapy)
• Drug-Eluting Stents
• Female
• Gene Expression Regulation
• Humans
• Inflammation Mediators (blood)
• Interleukin-1beta (blood)
• Interleukin-6 (blood)
• Ion Channel Gating
• Ion Channels (metabolism)
• Male
• Mammary Arteries (metabolism)
• Mechanotransduction, Cellular
• Middle Aged
• Muscle, Smooth, Vascular (metabolism)
• Myocytes, Smooth Muscle (metabolism)
• NF-kappa B (metabolism)
• Pressure
• Stress, Mechanical
• Time Factors