Abstract | CONTEXT: OBJECTIVES: MATERIALS AND METHODS: VSMCs, isolated from aortas of GK and non-diabetic rats, were cultured. The expression of proteins was evaluated by Western blot. The blood glucose concentration was measured with a blood glucose test meter. The diabetes of GK rats was controlled by blood glucose and insulin determinations (non-fasting values). The serum insulin concentration was determined by radioimmunoassay. RESULTS: In VSMCs from non-diabetic and GK rats, apocynin (1 and 10 µM) abolished the protein overexpression of NF-κB induced by glycated bovine serum albumin (AGEs-BSA) incubation. However, apocynin (1 and 10 µM) enhanced the expression of PPARγ protein in the presence of AGEs-BSA (100 μg/mL) in VSMCs from non-diabetic, but not from GK rats. CONCLUSION: These findings suggest that apocynin decreases the incidence of alterations in VSMCs induced by AGEs through the reduction of NF-κB and may represent an attractive therapeutic approach to treat diabetes mellitus.
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Authors | Cristina Oliveira Silva, Odair Alves da Silva, Gloria Pinto Duarte, Bernard Descomps, Saad Lahlou |
Journal | Pharmaceutical biology
(Pharm Biol)
Vol. 53
Issue 4
Pg. 488-93
(Apr 2015)
ISSN: 1744-5116 [Electronic] England |
PMID | 25471209
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acetophenones
- Antioxidants
- Glycation End Products, Advanced
- NF-kappa B
- PPAR gamma
- advanced glycation end products-bovine serum albumin
- Serum Albumin, Bovine
- acetovanillone
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Topics |
- Acetophenones
(pharmacology)
- Animals
- Antioxidants
(pharmacology)
- Aorta, Thoracic
(cytology, drug effects, metabolism)
- Blotting, Western
- Cell Culture Techniques
- Cells, Cultured
- Electrophoresis, Polyacrylamide Gel
- Glycation End Products, Advanced
(pharmacology)
- Male
- Muscle, Smooth, Vascular
(cytology, drug effects, metabolism)
- Myocytes, Smooth Muscle
(drug effects, metabolism)
- NF-kappa B
(biosynthesis)
- PPAR gamma
(biosynthesis)
- Rats, Inbred Strains
- Serum Albumin, Bovine
(pharmacology)
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