Abstract | BACKGROUND: METHODS: RESULTS: Treatment with formoterol and budesonide 72 h before and after RV14 infection reduced RV14 titers and cytokine concentrations, including interleukin (IL)-1β, IL-6 and IL-8, in supernatants and viral RNA within cells. Formoterol and budesonide reduced mRNA expression and protein concentration of intercellular adhesion molecule-1 (ICAM-1), the receptor for RV14. Formoterol reduced the number and fluorescence intensity of acidic endosomes through which RV RNA enters the cytoplasm. Formoterol and budesonide reduced the activation of the nuclear factor kappa-B protein p65 in nuclear extracts. The effects of formoterol plus budesonide were additive with respect to RV14 replication, cytokine production, ICAM-1 expression, acidic endosome fluorescence intensity, and p65 activation. The selective β(2)-adrenergic receptor antagonist, ICI 118551 [erythro-dl-1-(7-methylindan-4-yloxy)-3-isopropylaminobutan-2-ol], reversed the inhibitory effects of formoterol on RV14 titers and RNA levels, the susceptibility of cells to RV14 infection, cytokine production, acidic endosomes, ICAM-1 expression, and p65 activation. CONCLUSIONS:
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Authors | Mutsuo Yamaya, Hidekazu Nishimura, Lusamba Nadine, Hiroshi Kubo, Ryoichi Nagatomi |
Journal | Respiratory investigation
(Respir Investig)
Vol. 52
Issue 4
Pg. 251-60
(Jul 2014)
ISSN: 2212-5353 [Electronic] Netherlands |
PMID | 24998372
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Adrenergic beta-2 Receptor Agonists
- Cytokines
- Ethanolamines
- Glucocorticoids
- RNA, Viral
- Transcription Factor RelA
- Intercellular Adhesion Molecule-1
- Budesonide
- Formoterol Fumarate
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Topics |
- Adrenergic beta-2 Receptor Agonists
(pharmacology)
- Aged
- Budesonide
(pharmacology)
- Cells, Cultured
- Cytokines
(metabolism)
- Epithelial Cells
(metabolism, virology)
- Ethanolamines
(pharmacology)
- Female
- Formoterol Fumarate
- Glucocorticoids
(pharmacology)
- Humans
- Intercellular Adhesion Molecule-1
(metabolism)
- Male
- Picornaviridae Infections
(virology)
- RNA, Viral
(metabolism)
- Rhinovirus
(genetics, pathogenicity, physiology)
- Trachea
(cytology)
- Transcription Factor RelA
(metabolism)
- Virus Replication
(drug effects)
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