Oxygen nano-bubble water reduces calcium oxalate deposits and tubular cell injury in ethylene glycol-treated rat kidney.

Abstract

Renal tubular cell injury induced by oxalate plays an important role in kidney stone formation. Water containing oxygen nano-bubbles (nanometer-sized bubbles generated from oxygen micro-bubbles; ONB) has anti-inflammatory effects. Therefore, we investigated the inhibitory effects of ONB water on kidney stone formation in ethylene glycol (EG)-treated rats. We divided 60 rats, aged 4 weeks, into 5 groups: control, the water-fed group; 100 % ONB, the 100 % ONB water-fed group; EG, the EG treated water-fed group; EG + 50 % ONB and EG + 100 % ONB, water containing EG and 50 % or 100 % ONB, respectively. Renal calcium oxalate (CaOx) deposition, urinary excretion of N-acetyl-β-D-glucosaminidase (NAG), and renal expression of inflammation-related proteins, oxidative stress biomarkers, and the crystal-binding molecule hyaluronic acid were compared among the 5 groups. In the control and 100 % ONB groups, no renal CaOx deposits were detected. In the EG + 50 % ONB and EG + 100 % ONB groups, ONB water significantly decreased renal CaOx deposits, urinary NAG excretion, and renal monocyte chemoattractant protein-1, osteopontin, and hyaluronic acid expression and increased renal superoxide dismutase-1 expression compared with the EG group. ONB water substantially affected kidney stone formation in the rat kidney by reducing renal tubular cell injury. ONB water is a potential prophylactic agent for kidney stones.

• Authors: Yasuhiko Hirose, Takahiro Yasui, Kazumi Taguchi, Yasuhiro Fujii, Kazuhiro Niimi, Shuzo Hamamoto, Atsushi Okada, Yasue Kubota, Noriyasu Kawai, Yasunori Itoh, Keiichi Tozawa, Shoichi Sasaki, Kenjiro Kohri
• Journal: Urolithiasis (Urolithiasis) Vol. 41 Issue 4 Pg. 279-94 (Aug 2013) ISSN: 2194-7236 [Electronic] Germany
• PMID: 23754513 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ccl2 protein, rat
• Chemokine CCL2
• Spp1 protein, rat
• Water
• Osteopontin
• Calcium Oxalate
• Sod1 protein, rat
• Superoxide Dismutase
• Superoxide Dismutase-1
• Ethylene Glycol
• Oxygen

Topics

• Animals
• Calcium Oxalate (metabolism)
• Chemokine CCL2 (genetics)
• Ethylene Glycol (toxicity)
• Gene Expression (drug effects)
• Kidney Calculi (metabolism, pathology, prevention & control)
• Kidney Tubules (drug effects, injuries, metabolism)
• Male
• Nephrolithiasis (metabolism, pathology, prevention & control)
• Osteopontin (genetics)
• Oxygen (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (genetics)
• Superoxide Dismutase-1
• Water (chemistry)