Inhalative vs. systemic IL-10 administration: differences in the systemic inflammatory response and end-organ inflammation following hemorrhagic shock.

Abstract

Interleukin-10 is known to modulate the systemic inflammatory response after trauma. This study investigates differences in the systemic and end-organ inflammation in animals treated with either inhalative or systemic IL-10 after experimental hemorrhagic shock (HS). Pressure controlled HS was performed in C57/BL6 mice for 1.5h (6 animals per group). Inhalative or systemic recombinant mouse IL-10 (50 μg/kg dissolved in 50 μl PBS) was administered after resuscitation. Animals were sacrificed after 4.5 or 22.5h of recovery. Serum levels of IL-6, IL-10, KC, MCP-1, and LBP were determined by ELISA. Pulmonary and liver inflammation was analyzed by standardized Myeloperoxidase (MPO) kits. Systemic and inhalative IL-10 administration affected the systemic inflammatory response as well as end-organ inflammation differently. Differences were obvious in the early (6h) but not later (24h) inflammatory phase. Systemic IL-10 application was associated with a decreased systemic inflammatory response as well as hepatic inflammation, whereas nebulized IL-10 solely reduced the pulmonary inflammation. Our study demonstrates that systemic and nebulized IL-10 administration differentially influenced the systemic cytokine response and end-organ inflammation. Early pulmonary but not hepatic protection appears to be possible by inhalative IL-10 application. Further studies are necessary to assess exact pathways.

• Authors: Roman Pfeifer, Philipp Lichte, Helen Schreiber, Richard M Sellei, Joachim Schmidt, Derek Dombroski, Hans-Christoph Pape, Philipp Kobbe
• Journal: Cytokine (Cytokine) Vol. 60 Issue 1 Pg. 266-70 (Oct 2012) ISSN: 1096-0023 [Electronic] England
• PMID: 22727902 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Acute-Phase Proteins
• Carrier Proteins
• Ccl2 protein, mouse
• Chemokine CCL2
• Interleukin-6
• Membrane Glycoproteins
• lipopolysaccharide-binding protein
• Interleukin-10
• Peroxidase

Topics

• Acute-Phase Proteins
• Administration, Inhalation
• Animals
• Carrier Proteins (blood)
• Chemokine CCL2 (blood)
• Enzyme-Linked Immunosorbent Assay
• Hepatitis (blood, complications, prevention & control)
• Inflammation (blood, complications, prevention & control)
• Injections, Intra-Arterial
• Interleukin-10 (administration & dosage, blood, pharmacology)
• Interleukin-6 (blood)
• Liver (drug effects, metabolism, pathology)
• Lung (drug effects, metabolism, pathology)
• Male
• Membrane Glycoproteins (blood)
• Mice
• Mice, Inbred C57BL
• Peroxidase (metabolism)
• Pneumonia (blood, complications, prevention & control)
• Shock, Hemorrhagic (complications)
• Time Factors