Alternative therapeutic advantages of catfish bile on atopic dermatitis: protection of T cell-mediated skin disease via antioxidant activities.

Abstract

OBJECTIVES:

In the present study, we aimed to examine the anti-atopic properties of bile from the cat fish, Silurus asotus, to determine its possible use as a pharmaceutical product.

METHODS:

The anti-atopic activities of cat fish bile were examined in a non-cell antioxidant, in-vitro assay (splenocytes and mast cells) and a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis-like mouse model.

RESULTS:

The results of these experiments revealed that Silurus asotus bile (SAB) scavenges radicals and protects proteins from superoxide attacks, suggesting that SAB suppresses the T helper (Th) type 2-skewed immune response. Th1/Th2 mRNA cytokines (interleukin (IL)-2, interferon (IFN)-γ and IL-4) from mouse splenocytes were effectively inhibited, and the release of β-hexosaminidase in RBL-2H3 mast cells was significantly suppressed by SAB. These results were supported by screening the Th1/Th2 cytokine mRNAs (IL-2, IFN-γ and IL-4) from lymph nodes in DNCB-treated mice. More dramatic results were observed in the histological changes at higher SAB concentrations (5%) compared to the therapeutic control, visualized using hematoxylin-eosin (H&E) staining.

CONCLUSIONS:

The results presented in this study suggest that SAB may provide functional advantages with regard to treating atopic dermatitis because of its antioxidant and immune-suppressive effects.

• Authors: Do Ik Lee, Yoon Joo Huh, Kwang Woo Hwang, YoungWook Choi, Jae-Seok Choi, Sam-Yong Han, Young-Soo Gyoung, Seong Soo Joo
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 63 Issue 10 Pg. 1327-35 (Oct 2011) ISSN: 2042-7158 [Electronic] England
• PMID: 21899549 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2011 The Authors. JPP © 2011 Royal Pharmaceutical Society.

Chemical References

• Antioxidants
• Biological Products
• Cytokines
• Dinitrochlorobenzene
• RNA, Messenger
• Superoxides
• beta-N-Acetylhexosaminidases

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Bile
• Biological Products (pharmacology, therapeutic use)
• Catfishes
• Complementary Therapies
• Cytokines (antagonists & inhibitors, genetics)
• Dermatitis, Atopic (immunology, metabolism, therapy)
• Dinitrochlorobenzene
• Lymph Nodes (drug effects, metabolism)
• Mast Cells (drug effects, metabolism)
• Mice
• Mice, Inbred BALB C
• Protein Carbonylation (drug effects)
• RNA, Messenger (metabolism)
• Skin (drug effects, pathology)
• Spleen (cytology, drug effects, metabolism)
• Superoxides (metabolism)
• T-Lymphocytes (metabolism)
• Th1 Cells (drug effects, metabolism)
• Th2 Cells (drug effects, metabolism)
• beta-N-Acetylhexosaminidases (antagonists & inhibitors)