Abstract | BACKGROUND AND AIM: METHODS: We studied Mongolian gerbils inoculated with wild-type H. pylori 7.13 for up to 48 weeks and then examined animals infected with oipA, babA, or alpAB isogenic mutants for 12 weeks. Mucosal IL-6 and IL-11 mRNA levels were measured using real-time reverse transcription-polymerase chain reactions. RESULTS: High levels of gastric mucosal IL-6 and IL-11 mRNA in gerbils infected with wild-type H. pylori were observed during the chronic phase of infection, reaching maximums at 12 and 6 months, respectively. Infection with oipA and babA mutants resulted in significantly reduced cytokine levels and inflammatory cell infiltrations compared to gerbils infected with wild-type strains, and this persisted throughout the observation period. The alpAB mutants did not infect gerbils. Mucosal IL-6 and IL-11 levels were significantly associated with the grade of inflammatory cell infiltration. CONCLUSIONS:
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Authors | Mitsushige Sugimoto, Tomoyuki Ohno, David Y Graham, Yoshio Yamaoka |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 26
Issue 11
Pg. 1677-84
(Nov 2011)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 21679252
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
Chemical References |
- Adhesins, Bacterial
- AlpA protein, Helicobacter pylori
- AlpB protein, Helicobacter pylori
- BabA protein, Helicobacter pylori
- Bacterial Outer Membrane Proteins
- Interleukin-11
- Interleukin-6
- OipA protein, Helicobacter pylori
- RNA, Messenger
- Virulence Factors
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Topics |
- Adhesins, Bacterial
(metabolism)
- Animals
- Bacterial Outer Membrane Proteins
(genetics, metabolism)
- Base Sequence
- Disease Models, Animal
- Gastric Mucosa
(immunology, microbiology, pathology)
- Gerbillinae
- Helicobacter Infections
(immunology, microbiology, pathology)
- Helicobacter pylori
(genetics, immunology, metabolism, pathogenicity)
- Interleukin-11
(genetics, metabolism)
- Interleukin-6
(genetics, metabolism)
- Male
- Molecular Sequence Data
- Mutation
- RNA, Messenger
(metabolism)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Time Factors
- Up-Regulation
- Virulence Factors
(metabolism)
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