Abstract | AIMS: METHODS AND RESULTS: Obese ob/ob and db/db mice were treated with eplerenone, a MR antagonist, for 3 weeks. 3T3-L1 adipocytes were treated with aldosterone or H2O2, with and without eplerenone or MR- siRNA. High levels of MR mRNA were detected in adipose tissue of obese ob/ob and db/db mice. Eplerenone treatment significantly reduced insulin resistance, suppressed macrophage infiltration and ROS production in adipose tissues, and corrected the mRNA levels of obesity-related genes in obese mice. In 3T3-L1 adipocytes, aldosterone and H2O2 increased intracellular ROS levels and MR blockade inhibited such increases. H2O2 and aldosterone resulted in dysregulation of mRNAs of various genes related to ROS and cytokines, whereas MR blockade corrected such changes. CONCLUSION:
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Authors | Ayumu Hirata, Norikazu Maeda, Aki Hiuge, Toshiyuki Hibuse, Koichi Fujita, Takuya Okada, Shinji Kihara, Tohru Funahashi, Iichiro Shimomura |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 84
Issue 1
Pg. 164-72
(Oct 01 2009)
ISSN: 1755-3245 [Electronic] England |
PMID | 19505930
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Mineralocorticoid Receptor Antagonists
- RNA, Messenger
- RNA, Small Interfering
- Reactive Oxygen Species
- Receptors, Mineralocorticoid
- Spironolactone
- Eplerenone
- NADPH Oxidases
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Topics |
- 3T3-L1 Cells
- Adipocytes
(metabolism)
- Adipose Tissue
(metabolism, pathology)
- Animals
- Eplerenone
- Insulin Resistance
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Obese
- Mineralocorticoid Receptor Antagonists
- NADPH Oxidases
(genetics)
- Obesity
(metabolism)
- RNA, Messenger
(analysis)
- RNA, Small Interfering
(genetics)
- Reactive Oxygen Species
(metabolism)
- Receptors, Mineralocorticoid
(genetics, physiology)
- Spironolactone
(analogs & derivatives, pharmacology)
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