Abstract |
The effects of X-ray and carbon ion irradiation on DNA and genes in head and neck carcinoma cells were examined. Four head and neck cancer cell lines ( squamous cell carcinoma, salivary gland cancer, malignant melanoma, normal keratinocyte) were treated with 1, 4, and 7 GyE of carbon ion, or 1, 4, and 8 Gy of X-ray, respectively. DNA and RNA in the treated cells were extracted and purified. PCR-LOH (polymerase chain reaction-loss of heterozygosity) analysis with 6 microsatellite regions on chromosome 17 was performed to determine DNA structural damage, and then microarray analysis was performed to reveal changes in gene expression. PCR-LOH analysis detected high LOH in cells treated by radiation, indicating that most of the damage by X-ray occurred in the target region on one of the homologous chromosomes. However, carbon ion caused homo-deletion, which means deletion of the counterparts in both homologous chromosomes.
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Authors | Nobuharu Yamamoto, Chihaya Ikeda, Takashi Yakushiji, Takeshi Nomura, Akira Katakura, Takahiko Shibahara, Jun-Etsu Mizoe |
Journal | The Bulletin of Tokyo Dental College
(Bull Tokyo Dent Coll)
Vol. 48
Issue 4
Pg. 177-85
(Nov 2007)
ISSN: 0040-8891 [Print] Japan |
PMID | 18360104
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carbon Radioisotopes
- DNA, Neoplasm
- RNA, Neoplasm
- Radiopharmaceuticals
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Topics |
- Carbon Radioisotopes
(therapeutic use)
- Carcinoma, Squamous Cell
(genetics, radiotherapy)
- Cell Line
- Cell Line, Tumor
- Cell Survival
- Chromosomes, Human, Pair 17
(radiation effects)
- DNA, Neoplasm
(radiation effects)
- Gene Deletion
- Head and Neck Neoplasms
(genetics, radiotherapy)
- Humans
- Keratinocytes
(radiation effects)
- Loss of Heterozygosity
(radiation effects)
- Melanoma
(genetics, radiotherapy)
- Microsatellite Instability
(radiation effects)
- Microsatellite Repeats
(radiation effects)
- RNA, Neoplasm
(radiation effects)
- Radiopharmaceuticals
(therapeutic use)
- Radiotherapy Dosage
- Salivary Gland Neoplasms
(genetics, radiotherapy)
- Sequence Homology
- X-Rays
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