Abstract | OBJECTIVE: To observe the expression of thyroid transcription factor-1 (TTF-1) mRNA in human normal adult type II alveolar epithelial cells, embryonic alveolar epithelial cells, and primary lung carcinoma and lymph nodes, thereby exploring the role of TTF-1 mRNA expression in the tumorigenesis, development and metastasis of lung carcinoma. METHODS: TTF-1 mRNA was detected using tissue microarray and in situ hybridization in 1320 different paraffin-embedded tissue specimens. The intensity of TTF-1 mRNA in these tissues was assessed quantitatively using Leica Q500MC image analysis system. RESULTS: TTF-1 mRNA expression was significantly less intense in embryonic lung than in normal adult lung tissues (P= 0.000), and the two tissues both had significantly greater expression than lung adenocarcinoma, squamous cell carcinoma, small cell carcinoma and large cell carcinoma (P=0.000). Lung adenocarcinoma and small cell carcinoma, with similar expression intensity (P= 0.068), showed stronger expression than squamous cell carcinoma and large cell carcinoma (P=0.000), and squamous cell carcinoma showed stronger expression than large cell carcinoma (P=0.018). In lung adenocarcinoma, squamous cell carcinoma and large cell carcinoma, the intensity of TTF-1 mRNA expression was stronger in lymph node metastases than in the primary foci (P=0.003, P=0.000, P=0.019, respectively). The lymph node metastases had more intense expression than the primary foci of small cell lung carcinoma (P=0.078). The intensity of TTF-1 mRNA expression was greater in primary lung carcinomas with lymph node metastases than in those without metastases (P=0.026). Tumors of TNM stage II-IV had stronger expression than those of stage I (P=0.010). The intensity of TTF-1 mRNA expression was not associated with the patients' gender or the general types and differentiation of the tumor. CONCLUSION:
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Authors | Xiao-Yan Bai, Hong Shen |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 28
Issue 1
Pg. 20-5
(Jan 2008)
ISSN: 1673-4254 [Print] China |
PMID | 18227018
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- NKX2-1 protein, human
- Nuclear Proteins
- RNA, Messenger
- Thyroid Nuclear Factor 1
- Transcription Factors
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Topics |
- Adenocarcinoma
(genetics, pathology)
- Carcinoma, Squamous Cell
(genetics, pathology)
- Female
- Humans
- In Situ Hybridization
- Lung Neoplasms
(genetics, pathology)
- Lymphatic Metastasis
- Male
- Nuclear Proteins
(genetics)
- RNA, Messenger
(biosynthesis, genetics)
- Thyroid Gland
(metabolism)
- Thyroid Nuclear Factor 1
- Tissue Array Analysis
(methods)
- Transcription Factors
(genetics)
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