Differential expression of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in non-melanoma skin cancer: implications for tumour progression.

Abstract

AIMS:

To investigate the expression of matrix metalloproteinase (MMP)-2, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 in non-melanoma skin cancer (NMSC) and to compare their expression between different tumour types and with clinicopathological factors.

METHODS AND RESULTS:

A study of 11 normal skin, 29 Bowen's disease (BD), 40 squamous cell carcinoma (SCC) and 38 basal cell carcinoma (BCC) samples for MMP-2, MMP-9, TIMP-1 and TIMP-2 expression was carried out using immunohistochemistry and in situ hybridization. The expression of all metalloproteinases was greater in tumours than in normal skin. MMP-2 and MMP-9 expression was more extensive in the stroma of SCC than of BCC or BD. TIMP-1 expression was greater in the stroma of BCC than of SCC or BD and TIMP-2 expression was greater in the stroma of SCC than of BD. There was a correlation between increased metalloproteinase expression and depth of lesion (MMP-2 and TIMP-2), inflammation (MMP-2, MMP-9, TIMP-1 and TIMP-2) and microvessel density (MMP-2, MMP-9 and TIMP-2).

CONCLUSIONS:

MMP-2, MMP-9, TIMP-1 and TIMP-2 play an important role in the pathogenesis of non-melanoma skin cancer, but differ significantly in their expression levels between the tumour types examined. The immunoexpression of these proteins may be useful indicators of cutaneous cancer invasion and progression.

• Authors: A O'Grady, C Dunne, P O'Kelly, G M Murphy, M Leader, E Kay
• Journal: Histopathology (Histopathology) Vol. 51 Issue 6 Pg. 793-804 (Dec 2007) ISSN: 0309-0167 [Print] England
• PMID: 18042068 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Tissue Inhibitor of Metalloproteinase-1
• Tissue Inhibitor of Metalloproteinase-2
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Biomarkers, Tumor (analysis)
• Bowen's Disease (metabolism, pathology)
• Carcinoma, Basal Cell (metabolism, pathology)
• Carcinoma, Squamous Cell (metabolism, pathology)
• Disease Progression
• Gene Expression
• Humans
• Immunohistochemistry
• In Situ Hybridization
• Matrix Metalloproteinase 2 (biosynthesis)
• Matrix Metalloproteinase 9 (biosynthesis)
• Skin Neoplasms (metabolism, pathology)
• Tissue Inhibitor of Metalloproteinase-1 (biosynthesis)
• Tissue Inhibitor of Metalloproteinase-2 (biosynthesis)