Abstract | AIM/HYPOTHESIS: METHODS: Adipose tissue biopsies were taken from obese subjects (n = 44) with a wide range of insulin resistance, before and just after a 10-wk hypocaloric diet. ATGL and HSL protein and mRNA expression was determined by Western blot and quantitative RT-PCR, respectively. RESULTS: Fasting insulin levels and the degree of insulin resistance (using the homeostasis model assessment index for insulin resistance) were negatively correlated with ATGL and HSL protein expression, independent of age, gender, fat cell size, and body composition. Both mRNA and protein levels of ATGL and HSL were reduced in insulin-resistant compared with insulin-sensitive subjects (P < 0.05). Weight reduction significantly decreased ATGL and HSL mRNA and protein expression. A positive correlation between the decrease in leptin and the decrease in ATGL protein level after weight reduction was observed. Finally, ATGL and HSL mRNA and protein levels seem to be highly correlated, indicating a tight coregulation and transcriptional control. CONCLUSIONS:
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Authors | Johan W E Jocken, Dominique Langin, Egbert Smit, Wim H M Saris, Carine Valle, Gabby B Hul, Cecilia Holm, Peter Arner, Ellen E Blaak |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 92
Issue 6
Pg. 2292-9
(Jun 2007)
ISSN: 0021-972X [Print] United States |
PMID | 17356053
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Messenger
- Sterol Esterase
- Lipase
|
Topics |
- Adipose Tissue
(enzymology, pathology)
- Adult
- Biopsy
- Diet, Reducing
- Female
- Gene Expression Regulation, Enzymologic
- Humans
- Hyperinsulinism
(diet therapy, metabolism, pathology)
- Insulin Resistance
(physiology)
- Lipase
(genetics, metabolism)
- Male
- Middle Aged
- Obesity
(diet therapy, metabolism, pathology)
- RNA, Messenger
(metabolism)
- Regression Analysis
- Sterol Esterase
(genetics, metabolism)
- Weight Loss
(physiology)
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