Abstract | OBJECTIVE: METHODS: Sixty children were divided into ACD group (20 children), non- anemia (NA) group (19 children) and iron deficiency anemia (IDA) group (21 children) according to clinical diagnosis. Serum TNF alpha and IL-6 levels were detected with ELISA method. The EPO level was detected by chemical immulite method. The effect of rhTNF alpha on the expression of EPO gene was studied by culturing Hep G2 cell line and RT-PCR method. RESULTS: Serum EPO levels were different among the 3 groups (F = 44.68, P < 0.01). Serum EPO levels in ACD group were higher than those in NA group, while the hemoglobin levels were similar between the two groups. Serum EPO levels in ACD patients were lower than those in IDA patients. Serum TNF alpha levels were different among the 3 groups (F = 25.15, P < 0.01), and serum IL-6 levels were also different among the 3 groups (F = 13.16, P < 0.01). Serum TNF alpha and IL-6 levels in ACD group were higher than those in NA group. In ACD group, serum levels of both TNF alpha and IL-6 were not correlated to the serum level of EPO (r = -0.35, P > 0.05 and r = -0.05, P > 0.05, respectively). In vitro, rhTNF alpha inhibited the expression of EPO mRNA in hypoxia, and the inhibitory effects became stronger with the increase of rhTNF alpha (F = 64.20, P < 0.01). CONCLUSION: EPO levels increased incompensatively in ACD children, which may be a cause of ACD. TNF alpha may cause anemia by inhibiting EPO production.
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Authors | Xiao-wen Zhai, Yue Wu, Xiao-feng Gu, Feng-juan Lu |
Journal | Zhonghua er ke za zhi = Chinese journal of pediatrics
(Zhonghua Er Ke Za Zhi)
Vol. 42
Issue 1
Pg. 62-5
(Jan 2004)
ISSN: 0578-1310 [Print] China |
PMID | 14990112
(Publication Type: Comparative Study, English Abstract, Journal Article)
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Chemical References |
- Interleukin-6
- RNA, Messenger
- Recombinant Proteins
- Tumor Necrosis Factor-alpha
- Erythropoietin
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Topics |
- Anemia
(blood, genetics)
- Cell Line, Tumor
(drug effects, metabolism)
- Child
- Child, Preschool
- Chronic Disease
- Erythropoietin
(blood, genetics)
- Gene Expression
(drug effects)
- Humans
- Interleukin-6
(blood, genetics)
- RNA, Messenger
(genetics, metabolism)
- Recombinant Proteins
(pharmacology)
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Necrosis Factor-alpha
(genetics, metabolism, pharmacology)
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