Abstract | OBJECTIVE: Identification of leukemia-associated antigens (LAA) eliciting an immune response in patients is a prerequisite for specific immunotherapy of leukemias. To identify new LAA, we used the method of serologic screening of cDNA expression libraries (SEREX). MATERIALS AND METHODS: RESULTS: The receptor for hyaluronan acid-mediated motility (RHAMM) involved in cell growth and metastasis was identified as a new LAA. Serologic responses to RHAMM were observed in patients with AML (42%), CML (31%), melanoma (83%), renal cell carcinoma (40%), breast cancer (67%), and ovarian carcinoma (50%), but not in HV or patients with autoimmune diseases. RHAMM mRNA was detectable in peripheral blood mononuclear cells (PBMN) of 60% of newly diagnosed AML patients. Western blotting stained positive for RHAMM protein in 70% of AML patients. mRNA expression of RHAMM also was found in patients with CML (40%), renal cell carcinoma (73%), breast carcinoma (60%), and ovarian carcinoma (50%). In melanoma, RHAMM mRNA expression was detected in metastases (80%) but not in primary tumors. RHAMM is differentially expressed: significant mRNA expression was not found in normal tissues, except from testis, placenta, and thymus, or in PBMN- and CD34-separated cell samples of healthy volunteers. CONCLUSIONS:
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Authors | Jochen Greiner, Mark Ringhoffer, Masanori Taniguchi, Anita Schmitt, Dieter Kirchner, Gertraud Krähn, Volker Heilmann, Jürgen Gschwend, Lothar Bergmann, Hartmut Döhner, Michael Schmitt |
Journal | Experimental hematology
(Exp Hematol)
Vol. 30
Issue 9
Pg. 1029-35
(Sep 2002)
ISSN: 0301-472X [Print] Netherlands |
PMID | 12225794
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Neoplasm
- Antigens, Neoplasm
- Extracellular Matrix Proteins
- Hyaluronan Receptors
- Neoplasm Proteins
- RNA, Messenger
- RNA, Neoplasm
- hyaluronan-mediated motility receptor
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Topics |
- Acute Disease
- Antibodies, Neoplasm
(immunology)
- Antibody Specificity
- Antigens, Neoplasm
(biosynthesis, genetics, immunology)
- Breast Neoplasms
(metabolism)
- Carcinoma
(metabolism)
- Computer Systems
- Extracellular Matrix Proteins
(biosynthesis, genetics, immunology)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Hyaluronan Receptors
(biosynthesis, genetics, immunology)
- K562 Cells
(metabolism)
- Kidney Neoplasms
(metabolism)
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
(immunology, metabolism)
- Leukemia, Myeloid
(immunology, metabolism)
- Male
- Melanoma
(metabolism, pathology)
- Monocytes
(metabolism)
- Neoplasm Metastasis
- Neoplasm Proteins
(biosynthesis, genetics, immunology)
- Neoplasms
(metabolism)
- Neoplastic Stem Cells
(metabolism)
- Organ Specificity
- Ovarian Neoplasms
(metabolism)
- RNA, Messenger
(biosynthesis)
- RNA, Neoplasm
(biosynthesis)
- Reverse Transcriptase Polymerase Chain Reaction
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